Publication:
Immunochemical analysis and immunocytochemical localization of crustacean hyperglycemic hormone from the eyestalk of Macrobrachium rosenbergii

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Issued Date

1999

Resource Type

Article

File Type

application/pdf

ISSN

3050491

DOI

10.1016/S0305-0491(99)00100-5

Other identifier(s)

2-s2.0-0032845391

Rights Holder(s)

Scopus

Bibliographic Citation

Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. Vol 124, No.1 (1999), p.73-80

Suggested Citation

Sithigorngul P., Panchan N., Vilaivan T., Sithigorngul W., Petsom A. Immunochemical analysis and immunocytochemical localization of crustacean hyperglycemic hormone from the eyestalk of Macrobrachium rosenbergii. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. Vol 124, No.1 (1999), p.73-80. doi:10.1016/S0305-0491(99)00100-5 Retrieved from: https://hdl.handle.net/20.500.14740/7167

Title

Immunochemical analysis and immunocytochemical localization of crustacean hyperglycemic hormone from the eyestalk of Macrobrachium rosenbergii

Author(s)

Sithigorngul P.
 Panchan N.
 Vilaivan T.
 Sithigorngul W.
 Petsom A.

Abstract

Heptapeptide (YANAVQV-NH2=T-) and octapeptide (YANAVQTV-NH2=T+), the putative C-terminus of crustacean hyperglycemic hormone (CHH) from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii, was synthesized by solid phase peptide synthesis and conjugated to bovine serum albumin, then used for immunization in swiss mice. Specificity of the antisera against both peptides was determined by indirect immunoperoxidase ELISA. The best response of antiserum against each peptide was used to determine the presence of the natural CHH in the eyestalk extract after separation by one step of RP-HPLC using dot-ELISA. The peptide immunoreactive substances were found in fraction 30 using anti-T- antiserum and in fraction 38 using anti-T+ antiserum. However, the CHH activity was found only in fractions 37-39. Immunocytochemical localization of peptide immunoreactive substances in the eyestalk of M. rosenbergii using the anti-T- antiserum did not show any specific staining. In contrast, the anti-T+ antiserum revealed specific staining on a group of 24±5 neurons in medulla terminalis ganglionic x-organ and their processes through the sinus gland. Similar results were also obtained using the eyestalk of another species, the giant tiger prawn Penaeus monodon, in which 34±4 neuronal cells were recognized. These results strongly indicate that the anti-T+ antibody can bind to the natural CHH while the anti-T- antibody can not; therefore, this isoform of CHH in M. rosenbergii should consist of 72 residues and threonine is predicted to be present at position 71. Copyright (C) 1999 Elsevier Science Inc.

Subject(s)

Glucagon
 Animal experiment
 Animal tissue
 Antibody production
 Article
 Controlled study
 Crustacea
 Enzyme linked immunosorbent assay
 Hormone determination
 Hormone release
 Hormone synthesis
 Immunochemistry
 Immunocytochemistry
 Mouse
 Nonhuman
 Peptide synthesis
 Priority journal
 Bovinae
 Crustacea
 Macrobrachium rosenbergii
 Macrobrachium rosenbergii
 Monodon
 Panthera tigris
 Penaeus monodon
 Penaeus monodon
 Terminalis

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https://hdl.handle.net/20.500.14740/7167

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