Publication: Vasorelaxant and antioxidant activities of spilanthes acmella Murr
2
0
Issued Date
2008
Resource Type
File Type
application/pdf
ISSN
14220067
Other identifier(s)
2-s2.0-58149229380
Rights Holder(s)
Scopus
Bibliographic Citation
International Journal of Molecular Sciences. Vol 9, No.12 (2008), p.2724-2744
Suggested Citation
Wongsawatkul O., Prachayasittikul S., Isarankura-Na-Ayudhya C., Satayavivad J., Ruchirawat S., Prachayasittikul V. Vasorelaxant and antioxidant activities of spilanthes acmella Murr. International Journal of Molecular Sciences. Vol 9, No.12 (2008), p.2724-2744. doi:10.3390/ijms9122724 Retrieved from: https://hdl.handle.net/20.500.14740/3778
Abstract
This study reports the effect of Spilanthes acmella Murr. extracts on phenylephrine-induced contraction of rat thoracic aorta as well as their antioxidant activity. Results show that the extracts exert maximal vasorelaxations in a dose-dependent manner, but their effects are less than acetylcholine-induced nitric oxide (NO) vasorelaxation. Significant reduction of vasorelaxations is observed in both N G-nitro-L-arginine methyl ester (L-NAME) and indomethacin (INDO). In the presence of L-NAME plus INDO, synergistic effects are observed, leading to loss of vasorelaxation of both acetylcholine and the extracts. Similarly, the vasorelaxations of the extracts are completely abolished upon the removal of endothelial cells. This demonstrates that the extracts exhibit vasorelaxation via partially endothelium-induced NO and prostacyclin in a dose-dependent manner. Significantly, the ethyl acetate extract exerts immediate vasorelaxation (ED 50 76.1 ng/mL) and is the most potent antioxidant (DPPH assay). The chloroform extract shows the highest vasorelaxation and antioxidation (SOD assay). These reveal a potential source of vasodilators and antioxidants. © 2008.
Subject(s)
1,1 diphenyl 2 picrylhydrazyl
Acetic acid ethyl ester
Acetylcholine
Chloroform
Indometacin
N(g) nitroarginine methyl ester
Nitric oxide
Phenylephrine
Plant extract
Prostacyclin
Spilanthes acmella extract
Unclassified drug
Animal tissue
Antioxidant activity
Article
Endothelium cell
Extract
Medicinal plant
Nonhuman
Rat
Spilanthes acmella
Thoracic aorta
Vasodilatation
Rattus
Spilanthes acmella
Acetic acid ethyl ester
Acetylcholine
Chloroform
Indometacin
N(g) nitroarginine methyl ester
Nitric oxide
Phenylephrine
Plant extract
Prostacyclin
Spilanthes acmella extract
Unclassified drug
Animal tissue
Antioxidant activity
Article
Endothelium cell
Extract
Medicinal plant
Nonhuman
Rat
Spilanthes acmella
Thoracic aorta
Vasodilatation
Rattus
Spilanthes acmella