Publication:
Characterization of mucus-associated proteins from abalone (Haliotis) - Candidates for chemical signaling

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

1742464X

DOI

10.1111/j.1742-4658.2011.08436.x

Other identifier(s)

2-s2.0-84856096840

Rights Holder(s)

Scopus

Bibliographic Citation

FEBS Journal. Vol 279, No.3 (2012), p.437-450

Suggested Citation

Kuanpradit C., Stewart M.J., York P.S., Degnan B.M., Sobhon P., Hanna P.J., Chavadej J., Cummins S.F. Characterization of mucus-associated proteins from abalone (Haliotis) - Candidates for chemical signaling. FEBS Journal. Vol 279, No.3 (2012), p.437-450. doi:10.1111/j.1742-4658.2011.08436.x Retrieved from: https://hdl.handle.net/20.500.14740/7112

Title

Characterization of mucus-associated proteins from abalone (Haliotis) - Candidates for chemical signaling

Author(s)

Kuanpradit C.
 Stewart M.J.
 York P.S.
 Degnan B.M.
 Sobhon P.
 Hanna P.J.
 Chavadej J.
 Cummins S.F.

Abstract

Living in groups is a widespread phenomenon in the animal kingdom. For free-spawning aquatic animals, such as the abalone (Haliotis), being in the close proximity to potential mating partners enhances reproductive success. In this study, we investigated whether chemical cues could be present in abalone mucus that enable species-specific aggregation. A comparative MS analysis of mucus obtained from trailing or fixed stationary Haliotis asinina, and from seawater surrounding aggregations, indicated that water-soluble biomolecules are present and that these can stimulate sensory activity in conspecifics. Purified extracts of trail mucus contain at least three small proteins [termed H. asinina mucus-associated proteins (Has-MAPs)-1-3], which readily diffuse into the surrounding seawater and evoke a robust cephalic tentacle response in conspecifics. Mature Has-MAP-1 is approximately 9.9 kDa in size, and has a glycine-rich N-terminal region. Has-MAP-2 is approximately 6.2 kDa in size, and has similarities to schistosomin, a protein that is known to play a role in mollusc reproduction. The mature Has-MAP-3 is approximately 12.5 kDa in size, and could only be identified within trail mucus of animals outside of the reproductive season. All three Has-MAP genes are expressed at high levels within secretory cells of the juvenile abalone posterior pedal gland, consistent with a role in scent marking. We infer from these results that abalone mucus-associated proteins are candidate chemical cues that could provide informational cues to conspecifics living in close proximity and, given their apparent stability and hydrophilicity, animals further afield. © 2011 FEBS. No claim to original Australian government works.

Subject(s)

Glycine
 Mucus associated protein 1
 Mucus associated protein 2
 Mucus associated protein 3
 Secretory protein
 Unclassified drug
 Abalone
 Amino acid sequence
 Amino terminal sequence
 Animal experiment
 Animal tissue
 Article
 Chemical cue
 Controlled study
 Female
 Gene
 Gene expression regulation
 Gene location
 Gene sequence
 Haliotis asinina
 Hydrophilicity
 Juvenile animal
 Male
 Mass spectrometry
 Molecular recognition
 Molecular weight
 Mollusc larva
 Mucus associated protein gene
 Mucus secretion
 Nonhuman
 Nucleotide sequence
 Priority journal
 Protein aggregation
 Protein analysis
 Protein expression
 Protein function
 Protein secretion
 Protein stability
 Reproductive success
 Secretory cell
 Signal transduction
 Animals
 Mollusca
 Mucus
 Pheromones
 Proteins
 Reproduction
 Signal Transduction
 Species Specificity
 Animalia
 Haliotidae
 Haliotis
 Haliotis asinina
 Mollusca

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URI

https://hdl.handle.net/20.500.14740/7112

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Scopus: Year 1983-2021

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