Publication:
Indole-3-acetic acid biosynthetic pathways in the basidiomycetous yeast Rhodosporidium paludigenum

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0

Issued Date

2016

Resource Type

Article

File Type

application/pdf

ISSN

3028933

DOI

10.1007/s00203-016-1202-z

Other identifier(s)

2-s2.0-84959093852

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Archives of Microbiology. Vol 198, No.5 (2016), p.429-437

Suggested Citation

Nutaratat P., Srisuk N., Arunrattiyakorn P., Limtong S. Indole-3-acetic acid biosynthetic pathways in the basidiomycetous yeast Rhodosporidium paludigenum. Archives of Microbiology. Vol 198, No.5 (2016), p.429-437. doi:10.1007/s00203-016-1202-z Retrieved from: https://hdl.handle.net/20.500.14740/5330

Title

Indole-3-acetic acid biosynthetic pathways in the basidiomycetous yeast Rhodosporidium paludigenum

Author(s)

Nutaratat P.
 Srisuk N.
 Arunrattiyakorn P.
 Limtong S.

Abstract

Microorganisms produce plant growth regulators, such as auxins, cytokinins and gibberellins, to promote plant growth. Auxins are a group of compounds with an indole ring that have a positive effect on plant growth. Indole-3-acetic acid (IAA) is a plant growth hormone classified as an indole derivative of the auxin family. IAA biosynthesis pathways have been reported and widely studied in several groups of bacteria. Only a few studies on IAA biosynthesis pathways have been conducted in yeast. This study aimed to investigate IAA biosynthesis pathways in a basidiomycetous yeast (Rhodosporidium paludigenum DMKU-RP301). Investigations were performed both with and without a tryptophan supplement. Indole compound intermediates were detected by gas chromatography–mass spectrometry. Indole-3-lactic acid and indole-3-ethanol were found as a result of the enzymatic reduction of indole-3-pyruvic acid and indole-3-acetaldehyde, in IAA biosynthesis via an indole-3-pyruvic acid pathway. In addition, we also found indole-3-pyruvic acid in culture supernatants determined by high-performance liquid chromatography. Identification of tryptophan aminotransferase activity supports indole-3-pyruvic acid-routed IAA biosynthesis in R. paludigenum DMKU-RP301. We hence concluded that R. paludigenum DMKU-RP301 produces IAA through an indole-3-pyruvic acid pathway. © 2016, Springer-Verlag Berlin Heidelberg.

Subject(s)

Indole 3 acetaldehyde
 Indole 3 lactic acid
 Indole derivative
 Indoleacetic acid
 Indolepyruvic acid
 Tryptophan
 Tryptophan aminotransferase
 Tryptophol
 Unclassified drug
 Indole derivative
 Indoleacetic acid
 Indoleacetic acid derivative
 Phytohormone
 Article
 Enzyme activity
 High performance liquid chromatography
 Mass fragmentography
 Nonhuman
 Priority journal
 Reduction
 Rhodosporidium
 Rhodosporidium paludigenum
 Supernatant
 Thailand
 Basidiomycetes
 Biosynthesis
 Metabolism
 Physiology
 Basidiomycota
 Biosynthetic Pathways
 Gas Chromatography-Mass Spectrometry
 Indoleacetic Acids
 Indoles
 Plant Growth Regulators
 Tryptophan

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URI

https://hdl.handle.net/20.500.14740/5330

Collections

Scopus: Year 1983-2021

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