Publication:
Preferential suppression of yellow head virus (YHV) envelope protein gp116 in shrimp that survive challenge with YHV

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Issued Date

2008

Resource Type

Article

File Type

application/pdf

ISSN

1775103

DOI

10.3354/dao01883

Other identifier(s)

2-s2.0-42249093009

Rights Holder(s)

Scopus

Bibliographic Citation

Diseases of Aquatic Organisms. Vol 79, No.1 (2008), p.1-8

Suggested Citation

Chaivisuthangkura P., Tejangkura T., Rukpratanporn S., Longyant S., Sithigorngul W., Sithigorngul P. Preferential suppression of yellow head virus (YHV) envelope protein gp116 in shrimp that survive challenge with YHV. Diseases of Aquatic Organisms. Vol 79, No.1 (2008), p.1-8. doi:10.3354/dao01883 Retrieved from: https://hdl.handle.net/20.500.14740/4102

Title

Preferential suppression of yellow head virus (YHV) envelope protein gp116 in shrimp that survive challenge with YHV

Author(s)

Chaivisuthangkura P.
 Tejangkura T.
 Rukpratanporn S.
 Longyant S.
 Sithigorngul W.
 Sithigorngul P.

Abstract

The DNA sequence that encodes the first 406 amino acid residues at the N-terminus of yellow head virus (YHV) protein gp116, namely N/2 gp116ΔTM, and the DNA sequence that encodes the next 392 amino acid residues at the C-terminus of gp116 (without the transmembrane region), namely C/2 gp116ΔTM, were cloned into pGEX-6P-1 plasmid and expressed in E. coli. Both recombinant proteins were expressed, purified by SDS-PAGE and used to immunize mice. The mouse anti-recombinant N/2 gp116 and C/2 gp116 antisera bound specifically to both the recombinant proteins and to natural gp116 protein in YHV-infected haemolymph as shown by Western blotting and in tissues as shown by immunohistochemistry. Immunohistochemical localization of YHV using anti-gp116 antiserum or monoclonal antibodies specific to gp116 (V3-2B), gp64 (Y18) and p20 (Y19) revealed similar immunoreactivity patterns for all these reagents in muscle and mandibular tissue in shrimp showing gross signs of yellow head disease. However, in gill, hepatopancreas, lymphoid organ and thoracic ganglion tissues from experimental YHV-infected shrimp (Penaeus vannamei and Palaemon serrifer) that did not show signs of disease, immunoreactivity to gp116 was reduced or absent while that for gp64 and p20 remained intense. Thus, some shrimp species were able to selectively inhibit the synthesis of gp116 in a manner that was associated with absence of gross signs of disease. © Inter-Research 2008.

Subject(s)

Antiserum
 Recombinant protein
 Virus antibody
 Virus envelope protein
 Amino acid
 Antibody
 Crustacean
 Immunoassay
 Protein
 Viral disease
 Animal
 Article
 Escherichia coli
 Female
 Gene expression regulation
 Genetics
 Immunohistochemistry
 Metabolism
 Mouse
 Nidovirales
 Palaemonidae
 Penaeidae
 Physiology
 Reverse transcription polymerase chain reaction
 Sensitivity and specificity
 Virology
 Western blotting
 Animals
 Antibodies, Viral
 Blotting, Western
 Escherichia coli
 Female
 Gene Expression Regulation, Viral
 Immune Sera
 Immunohistochemistry
 Mice
 Palaemonidae
 Penaeidae
 Recombinant Proteins
 Reverse Transcriptase Polymerase Chain Reaction
 Roniviridae
 Sensitivity and Specificity
 Viral Envelope Proteins
 Decapoda (Crustacea)
 Escherichia coli
 Litopenaeus vannamei
 Mus
 Palaemon serrifer
 Penaeus monodon
 Yellow head virus

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URI

https://hdl.handle.net/20.500.14740/4102

Collections

Scopus: Year 1983-2021

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