Publication:
Suppression of human fibrosarcoma cell metastasis by Phyllanthus emblica extract in vitro

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

15137368

DOI

10.7314/APJCP.2013.14.11.6863

Other identifier(s)

2-s2.0-84892456221

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Asian Pacific Journal of Cancer Prevention. Vol 14, No.11 (2013), p.6863-6867

Suggested Citation

Yahayo W., Supabphol A., Supabphol R. Suppression of human fibrosarcoma cell metastasis by Phyllanthus emblica extract in vitro. Asian Pacific Journal of Cancer Prevention. Vol 14, No.11 (2013), p.6863-6867. doi:10.7314/APJCP.2013.14.11.6863 Retrieved from: https://hdl.handle.net/20.500.14740/6492

Title

Suppression of human fibrosarcoma cell metastasis by Phyllanthus emblica extract in vitro

Author(s)

Yahayo W.
 Supabphol A.
 Supabphol R.

Abstract

Phyllanthus emblica (PE) is known to exhibit various pharmacological properties. This study aimed to evaluate the antimetastatic potential of a PE aqueous extract. Cytotoxicity to human fibrosarcoma cells, HT1080, was determined by viability assay using the 3-(4,5-dimethylthiazol,2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent. Cell migration and invasion were investigated using chemotaxis chambers containing membranes precoated with collagen IV and Matrigel, respectively. Cell attachment onto normal surfaces of cell culture plates was tested to determine the cell-adhesion capability. The molecular mechanism of antimetastatic activity was assessed by measuring the gene expression of matrix metalloproteinases, MMP2, and MMP9, using reverse transcription-polymerase chain reaction (RT-PCR) assay. The mRNA levels of both genes were significantly down-regulated after pretreatment with PE extract for 5 days. Our findings show the antimetastatic function of PE extract in reducing cell proliferation, migration, invasion, and adhesion in both dose- and time-dependent manners, especially growth arrest with low IC50 value. A decrease in the expression of both MMP2 and MMP9 seems to be the cellular mechanism for antimetastasis in this case. There is a high potential to use PE extracts clinically as an optional adjuvant therapeutic drug for therapeutic intervention strategies in cancer therapy or chemoprevention.

Subject(s)

Gelatinase A
 Gelatinase B
 Messenger RNA
 MMP2 protein, human
 MMP9 protein, human
 Plant extract
 Apoptosis
 Article
 Cell adhesion
 Cell motion
 Cell proliferation
 Chemistry
 Drug effect
 Emblica officinalis
 Fibrosarcoma
 Genetics
 Human
 In vitro study
 Metabolism
 Metastasis
 Phytotherapy
 Real time polymerase chain reaction
 Reverse transcription polymerase chain reaction
 Tumor cell culture
 Apoptosis
 Cell Adhesion
 Cell Movement
 Cell Proliferation
 Fibrosarcoma
 Humans
 Matrix Metalloproteinase 2
 Matrix Metalloproteinase 9
 Phyllanthus emblica
 Phytotherapy
 Plant Extracts
 Real-Time Polymerase Chain Reaction
 Reverse Transcriptase Polymerase Chain Reaction
 RNA, Messenger
 Tumor Cells, Cultured

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URI

https://hdl.handle.net/20.500.14740/6492

Collections

Scopus: Year 1983-2021

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