Publication:
Transspecies dimorphic allelic lineages of the proteasome subunit β-type 8 gene (PSMB8) in the teleost genus Oryzias

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

278424

DOI

10.1073/pnas.1012881107

Other identifier(s)

2-s2.0-78650733024

Rights Holder(s)

Scopus

Bibliographic Citation

Proceedings of the National Academy of Sciences of the United States of America. Vol 107, No.50 (2010), p.21599-21604

Suggested Citation

Miura F., Tsukamoto K., Mehta R.B., Naruse K., Magtoon W., Nonaka M. Transspecies dimorphic allelic lineages of the proteasome subunit β-type 8 gene (PSMB8) in the teleost genus Oryzias. Proceedings of the National Academy of Sciences of the United States of America. Vol 107, No.50 (2010), p.21599-21604. doi:10.1073/pnas.1012881107 Retrieved from: https://hdl.handle.net/20.500.14740/7437

Title

Transspecies dimorphic allelic lineages of the proteasome subunit β-type 8 gene (PSMB8) in the teleost genus Oryzias

Author(s)

Miura F.
 Tsukamoto K.
 Mehta R.B.
 Naruse K.
 Magtoon W.
 Nonaka M.

Abstract

The proteasome subunit β-type 8 (PSMB8) gene in the jawed vertebrate MHC genomic region encodes a catalytic subunit of the immunoproteasome involved in the generation of peptides to be presented by the MHC class I molecules. A teleost, the medaka (Oryzias latipes), has highly diverged dimorphic allelic lineages of the PSMB8 gene with only about 80% amino acid identity, termed "PSMB8d" and "PSMB8N," which have been retained by most wild populations analyzed. To elucidate the evolutionary origin of these two allelic lineages, seven species of the genus Oryzias were analyzed for their PSMB8 allelic sequences using a large number of individuals from wild populations. All the PSMB8 alleles of these species were classified into one of these two allelic lineages based on their nucleotide sequences of exons and introns, indicating that the Oryzias PSMB8 gene has a truly dichotomous allelic lineage. Retention of both allelic lineages was confirmed except for one species. The PSMB8d lineage showed a higher frequency than the PSMB8N lineage in all seven species. The two allelic lineages showed curious substitutions at the 31st and 53rd residues of the mature peptide, probably involved in formation of the S1 pocket, suggesting that these allelic lineages show a functional difference in cleavage specificity. These results indicate that the PSMB8 dimorphism was established before speciation within the genus Oryzias and has been maintained for more than 30-60 million years under a strict and asymmetric balancing selection through several speciation events.

Subject(s)

Major histocompatibility antigen class 1
 Proteasome subunit beta type 8
 Protein
 Unclassified drug
 Allele
 Amino acid analysis
 Amino acid sequence
 Amino acid substitution
 Article
 Evolution
 Exon
 Gene sequence
 Intron
 Nonhuman
 Nucleotide sequence
 Oryzias
 Priority journal
 Species differentiation
 Teleost
 Vertebrate
 Alleles
 Amino Acid Sequence
 Animals
 Base Sequence
 Evolution, Molecular
 Histocompatibility Antigens Class I
 Major Histocompatibility Complex
 Molecular Sequence Data
 Oryzias
 Phylogeny
 Polymorphism, Genetic
 Proteasome Endopeptidase Complex
 Protein Subunits
 Gnathostomata (vertebrate)
 Oryzias
 Oryzias latipes
 Oryziinae
 Teleostei

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URI

https://hdl.handle.net/20.500.14740/7437

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Scopus: Year 1983-2021

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