Publication: Antioxidant Properties of Unripe Carica papaya Fruit Extract and Its Protective Effects against Endothelial Oxidative Stress
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Issued Date
2019
Resource Type
File Type
application/pdf
ISSN
1741427X
Other identifier(s)
2-s2.0-85068591602
Rights Holder(s)
Scopus
Bibliographic Citation
Evidence-based Complementary and Alternative Medicine. Vol 2019, (2019)
Suggested Citation
Jarisarapurin W., Sanrattana W., Chularojmontri L., Kunchana K., Wattanapitayakul S.K. Antioxidant Properties of Unripe Carica papaya Fruit Extract and Its Protective Effects against Endothelial Oxidative Stress. Evidence-based Complementary and Alternative Medicine. Vol 2019, (2019). doi:10.1155/2019/4912631 Retrieved from: https://hdl.handle.net/20.500.14740/5609
Abstract
It has been proven that high consumption of fruit and vegetable lowers the risks of cardiovascular and other oxidative stress-related diseases. Here we evaluated the effects of a tropical fruit, unripe Carica papaya (UCP), on endothelial protection against oxidative damage induced by H2O2. The antioxidant properties of UCP were investigated using the assays of FRAP and ORAC and specific ROS scavenging activities (H2O2, O 2 • -, OH•, HOCl). Cytoprotective property was tested in human endothelial cell line EA.hy926 with respect to cell survival, intracellular ROS levels, antioxidant enzyme activities (CAT, SOD, GPX), survival/stress signaling (AKT, JNK, p38), and nuclear signaling (Nrf2, NF-kB). UCP processed high antioxidant activity and scavenging activity against H2O2> OH•> O 2 • - > HOCl, respectively. UCP improved cell survival in the milieu of ROS reduction. While SOD was increased by UCP, CAT activity was enhanced when cells were challenged with H2O2. UCP had no impact on H2O2-activated AKT, JNK, and p38 signaling but significantly decreased nuclear NF-B levels. The overactivation of Nrf2 in response to oxidative stress was constrained by UCP. In conclusion, UCP protected endothelial cells against oxidative damage through intracellular ROS reduction, enhanced CAT activity, suppression of NF-kB, and prohibition of Nrf2 dysregulation. Thus, UCP might be a candidate for development of nutraceuticals against CVD and oxidative-related diseases and conditions. © 2019 Wattanased Jarisarapurin et al.
Subject(s)
Carica papaya extract
Hydrogen peroxide
Immunoglobulin enhancer binding protein
Protein kinase B
Reactive oxygen metabolite
Stress activated protein kinase
Synaptophysin
Antioxidant activity
Article
Cell protection
Cell survival
Cell viability
Endothelium cell
Enzyme activation
Enzyme activity
Evaluation study
Human
Human cell
Oxidative stress
Signal transduction
Hydrogen peroxide
Immunoglobulin enhancer binding protein
Protein kinase B
Reactive oxygen metabolite
Stress activated protein kinase
Synaptophysin
Antioxidant activity
Article
Cell protection
Cell survival
Cell viability
Endothelium cell
Enzyme activation
Enzyme activity
Evaluation study
Human
Human cell
Oxidative stress
Signal transduction