Publication:
Penaeus monodon nucleopolyhedrovirus detection using an immunochromatographic strip test

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

1660934

DOI

10.1016/j.jviromet.2012.04.016

Other identifier(s)

2-s2.0-84862127708

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Virological Methods. Vol 183, No.2 (2012), p.210-214

Suggested Citation

Wangman P., Longyant S., Chaivisuthangkura P., Sridulyakul P., Rukpratanporn S., Sithigorngul P. Penaeus monodon nucleopolyhedrovirus detection using an immunochromatographic strip test. Journal of Virological Methods. Vol 183, No.2 (2012), p.210-214. doi:10.1016/j.jviromet.2012.04.016 Retrieved from: https://hdl.handle.net/20.500.14740/6988

Title

Penaeus monodon nucleopolyhedrovirus detection using an immunochromatographic strip test

Author(s)

Wangman P.
 Longyant S.
 Chaivisuthangkura P.
 Sridulyakul P.
 Rukpratanporn S.
 Sithigorngul P.

Abstract

An immunochromatographic strip test is described for detection of the polyhedrin protein of Penaeus monodon nucleopolyhedrovirus (PemoNPV). The test employs one monoclonal antibody (MAb MBV5) conjugated to colloidal gold to bind to polyhedrin protein and a 1:1:1 mixture of 3 other MAbs (MBV8, 14 and 21) to capture colloidal-gold MAb-protein complexes at a test (T) line on the nitrocellulose strip. A downstream control (C) line of goat anti-mouse immunoglobulin G (GAM) antibody is used to capture excess free colloidal-gold conjugated MBV5 to validate test performance. Heating of homogenates of PemoNPV-infected P. monodon postlarvae prepared in PBS for 30. min was necessary to maximize T line color intensity, and homogenates of infected postlarvae could still be scored as PemoNPV-positive when diluted 1:64. A strip test result was obtained within 15. min of sample application, and although about 200-fold lower than a one-step PCR test for PemoNPV, its detection sensitivity was comparable to a dot blot. Due to its simplicity not reliant on sophisticated equipment or specialized skills, the strip test could be adopted to screen easily for PemoNPV infections at shrimp hatcheries and farms. © 2012 Elsevier B.V.

Subject(s)

Immunoglobulin G antibody
 Monoclonal antibody
 Polyhedrin
 Animal experiment
 Animal model
 Article
 Controlled study
 Immunoaffinity chromatography
 Microbial sensitivity test
 Nonhuman
 Penaeus monodon
 Polyhedrosis virus
 Polymerase chain reaction
 Priority journal
 Protein binding
 Reovirus infection
 Sensitivity and specificity
 Task performance
 Validation process
 Virus detection
 Animals
 Antibodies, Immobilized
 Antibodies, Monoclonal, Murine-Derived
 Chromatography, Thin Layer
 DNA, Viral
 Hybridomas
 Immunologic Tests
 Larva
 Nucleopolyhedrovirus
 Penaeidae
 Sensitivity and Specificity
 Capra hircus
 Decapoda (Crustacea)
 Monodon baculovirus
 Nucleopolyhedrovirus
 Penaeus monodon

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URI

https://hdl.handle.net/20.500.14740/6988

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Scopus: Year 1983-2021

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