Publication: Vasorelaxation and superoxide scavenging activities of orotic acid
1
0
Issued Date
2010
Resource Type
File Type
application/pdf
ISSN
18117775
Other identifier(s)
2-s2.0-77955644960
Rights Holder(s)
Scopus
Bibliographic Citation
International Journal of Pharmacology. Vol 6, No.4 (2010), p.375-380
Suggested Citation
Prachayasittikul S., Wongsawatkul O., Worachartcheewan A., Ruchirawat S., Prachayasittikul V. Vasorelaxation and superoxide scavenging activities of orotic acid. International Journal of Pharmacology. Vol 6, No.4 (2010), p.375-380. Retrieved from: https://hdl.handle.net/20.500.14740/7551
Abstract
The aim of study is to investigate effects of orotic acid (OA) on phenylephrine-induced contraction of rat thoracic aorta and its antioxidative activity. Results showed that the OA exhibited maximal vasorelaxation in dose-dependent manner with ED50 of 3.1?×10-7 M, but the effect was less than those of acetylcholine (ACh)-induced nitric oxide (NO) vasorelaxation. Significant reductions of the vasorelaxations were found in the presence of either NG-nitro-L-arginine methyl ester (L-NAME) or indomethacin (INDO). Synergistic effects were observed in the presence of L-NAME plus INDO that led to loss of vasorelaxation of both the ACh and the OA. In addition, complete loss of the vasorelaxation was manifested under removal of endothelial cells. This implies that the vasorelaxations are mediated by partially endothelium-induced NO and prostacyclin. The OA exhibited antioxidative activity in both DPPH and SOD assays. The significant results reveal novel actions of the OA as vasorelaxants and superoxide scavenger which are benefits as therapeutic uses and health supplements. © 2010 Asian Network for Scientific Information.
Subject(s)
1,1 diphenyl 2 picrylhydrazyl
Acetylcholine
Indometacin
N(g) nitroarginine methyl ester
Nitric oxide
Orotic acid
Prostacyclin
Superoxide dismutase
Animal experiment
Animal tissue
Antioxidant activity
Aorta constriction
Article
Controlled study
Drug mechanism
Drug potentiation
Drug response
Drug structure
Endothelium cell
Male
Nonhuman
Rat
Thoracic aorta
Vasodilatation
Acetylcholine
Indometacin
N(g) nitroarginine methyl ester
Nitric oxide
Orotic acid
Prostacyclin
Superoxide dismutase
Animal experiment
Animal tissue
Antioxidant activity
Aorta constriction
Article
Controlled study
Drug mechanism
Drug potentiation
Drug response
Drug structure
Endothelium cell
Male
Nonhuman
Rat
Thoracic aorta
Vasodilatation