Publication:
The effect of temperature of eye irrigation solution to reduce corneal endothelial cell loss during phacoemulsification: An in vitro model study

0
0

Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84876941086

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S83-S89

Suggested Citation

Uthaisang-Tanechpongtamb W., Limtrakarn W., Reepolmaha S. The effect of temperature of eye irrigation solution to reduce corneal endothelial cell loss during phacoemulsification: An in vitro model study. Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S83-S89. Retrieved from: https://hdl.handle.net/20.500.14740/6874

Title

The effect of temperature of eye irrigation solution to reduce corneal endothelial cell loss during phacoemulsification: An in vitro model study

Author(s)

Uthaisang-Tanechpongtamb W.
 Limtrakarn W.
 Reepolmaha S.

Abstract

Objective: To compare the efficiency of eye irrigation solution (balanced salt solution: BSS) stored in a refrigerator and air-conditioned room to reduce the number of corneal endothelial cell loss during the process of phacoemulsification. Material and Method: Porcine corneal endothelial cells (PCEC) isolated from porcine eyes were used as a model. The porcine eyes were obtained from a local slaughterhouse within 6 h after death and PCEC were cultured as standard method. The percentage of cell survival was evaluated by MTT assay. Results: The PCEC was successfully grown in the standard culturing system. The growth curve of PCEC demonstrated that log phase was reached in 3-6 days. The evaluation of heat effect (40-60°C) to PCECsurvival was shown that cells death was found at 55 and 60°C which the percentage of cell survival reduced to 30% at 60°C, 180 sec. Further observation on the efficiency of BSS stored in different conditions, refrigeration (8°C) and air-conditioned room (25°C) to PCEC survival was revealed that the number of cell survival increased from 40% to almost 70% when using refrigerated BSS as irrigation solution, while from air-conditioned BSS only at 55%. Conclusion: From using PCEC as a model and mimic the process of irrigation in phacoemulsification, it was found that the eye irrigation solution (balanced salt solution: BSS) stored in the refrigerator had a better result in helping protection the corneal endothelial cell loss from heat damaged than BSS stored in air-conditioned room.

Subject(s)

Balanced salt solution
 Air conditioning
 Animal cell
 Animal model
 Article
 Cell death
 Cell growth
 Cell isolation
 Cell structure
 Cell survival
 Controlled study
 Cornea endothelium
 Corneal endothelial cell loss
 Eye lavage
 Freezing
 Heat
 Nonhuman
 Phacoemulsification
 Swine
 Temperature
 Analysis of Variance
 Animals
 Cell Death
 Cell Survival
 Cells, Cultured
 Corneal Endothelial Cell Loss
 Endothelium, Corneal
 Ophthalmic Solutions
 Phacoemulsification
 Swine
 Temperature
 Therapeutic Irrigation

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6874

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By