Publication:
Development of clock genes expression in rat hippocampus

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Issued Date

2015

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84957696511

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 98, (2015), p.S123-S129

Suggested Citation

Pramong R., Wongchitrat P., Govitrapong P., Phansuwan-Pujito P. Development of clock genes expression in rat hippocampus. Journal of the Medical Association of Thailand. Vol 98, (2015), p.S123-S129. Retrieved from: https://hdl.handle.net/20.500.14740/6067

Title

Development of clock genes expression in rat hippocampus

Author(s)

Pramong R.
 Wongchitrat P.
 Govitrapong P.
 Phansuwan-Pujito P.

Abstract

Background: The circadian rhythms in the suprachiasmatic nucleus (SCN), a central clock, are generated by autoregulatory network composed of clock genes that encode transcriptional factors. There is a gradual development of clock gene expression in the SCN during ontogenesis. Moreover, clock genes are expressed in the adult hippocampus with circadian fashion. Objective: It is of interest to examine daily profiles of the clock gene mRNA and protein expressions in rat hippocampus during development. Material and Method: Daily profiles of three clock genes (Per1, Per2, and Bmal1) mRNA, and their protein expressions were analyzed in the rat hippocampus of pups at postnatal (P) day 4 and 8 (P4 and P8), pre-weaning stage (P16), early pubertal stage (P32), and adult (P60) by real-time PCR and immunohistochemistry. Results: The entire studied clock gene mRNAs and proteins did not exhibit circadian rhythm in early postnatal P4-P16. Rhythmic expression of Per1 and Per2 mRNA started at P32, whereas Bmal1 began at adult. However, their proteins showed circadian expression together at adult. Conclusion: The present study suggests that rat hippocampal molecular clock works gradually develop after birth and slower than that in the central clock SCN. It was possible that ontogenetic development of clock gene in hippocampus was waiting for central clock synchronization. © 2015, Medical Association of Thailand. All rights reserved.

Subject(s)

Messenger RNA
 PER1 protein
 PER2 protein
 Transcription factor ARNTL
 Messenger RNA
 Animal model
 Animal tissue
 Article
 Brain growth
 Circadian rhythm
 Clock gene
 Controlled study
 Densitometry
 Female
 Gene
 Gene expression
 Genetic analysis
 Immunohistochemistry
 Immunoreactivity
 Microscopy
 Molecular clock
 Nonhuman
 Rat
 Real time polymerase chain reaction
 RNA analysis
 RNA isolation
 Animal
 Circadian rhythm
 Gene expression regulation
 Genetics
 Hippocampus
 Metabolism
 Suprachiasmatic nucleus
 Time
 Wistar rat
 Animals
 Circadian Rhythm
 Gene Expression Regulation, Developmental
 Hippocampus
 Rats
 Rats, Wistar
 Real-Time Polymerase Chain Reaction
 RNA, Messenger
 Suprachiasmatic Nucleus
 Time Factors

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https://hdl.handle.net/20.500.14740/6067

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Scopus: Year 1983-2021

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