Publication:
Silencing of a Kazal-type serine proteinase inhibitor SPIPm2 from Penaeus monodon affects YHV susceptibility and hemocyte homeostasis

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Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

10504648

DOI

10.1016/j.fsi.2018.05.004

Other identifier(s)

2-s2.0-85046674536

Rights Holder(s)

Scopus

Bibliographic Citation

Fish and Shellfish Immunology. Vol 79, (2018), p.18-27

Suggested Citation

Visetnan S., Donpudsa S., Tassanakajon A., Rimphanitchayakit V. Silencing of a Kazal-type serine proteinase inhibitor SPIPm2 from Penaeus monodon affects YHV susceptibility and hemocyte homeostasis. Fish and Shellfish Immunology. Vol 79, (2018), p.18-27. doi:10.1016/j.fsi.2018.05.004 Retrieved from: https://hdl.handle.net/20.500.14740/3732

Title

Silencing of a Kazal-type serine proteinase inhibitor SPIPm2 from Penaeus monodon affects YHV susceptibility and hemocyte homeostasis

Author(s)

Visetnan S.
 Donpudsa S.
 Tassanakajon A.
 Rimphanitchayakit V.

Abstract

In shrimp, the Kazal-type serine proteinase inhibitors (KPIs) are involved in host innate immune defense system against pathogenic microorganisms. A five-Kazal-domain SPIPm2 is the most abundant KPIs in the black tiger shrimp Penaeus monodon and up-regulated in response to yellow head virus (YHV) infection. In this study, the role of SPIPm2 in YHV infection was investigated. The expression of SPIPm2 in hemocytes, gill and heart from 48-h YHV-infected shrimp was increased. The expression of SPIPm2 in hemocytes was significantly increased after 12 h of infection and gradually increased higher afterwards. Silencing of SPIPm2 by dsRNA interference resulted in the increased expression of different apoptosis-related genes, the increased expression of transcriptional factors of antimicrobial synthesis pathways, the reduction of circulating hemocytes in the shrimp hemolymph, and the increased susceptibility of the silenced shrimp to YHV infection. The activities of caspase-3 and caspase-7 in the hemocytes of SPIPm2-silenced shrimp was also increased by 5.32-fold as compared with those of the control shrimp. The results suggested that the SPIPm2 was involved in the hemocyte homeostasis. © 2018

Subject(s)

ALFPm3 protein
 Arthropod protein
 Caspase 3
 Caspase 7
 CrustinPm1 protein
 CrustinPm7 protein
 Cyclophilin A
 Double stranded RNA
 Kazal type serine proteinase inhibitor SPIPm2
 Penaeidin3 protein
 Penaeidin5 protein
 PmDorsal protein
 PmHHAP protein
 PmIAP protein
 PmKunitz protein
 PmPO1 protein
 PmPO2 protein
 PmPPAE1 protein
 PmPPAE2 protein
 PmRelish protein
 PmToll protein
 Serine peptidase inhibitor Kazal type
 SPIPm5 protein
 SWDPm2 protein
 Transcription factor
 Unclassified drug
 Arthropod protein
 Serine peptidase inhibitor Kazal type
 Animal experiment
 Animal tissue
 Apoptosis
 Article
 Blood cell
 Controlled study
 Disease predisposition
 Down regulation
 Enzyme activity
 Gene expression
 Gene knockdown
 Gene silencing
 Gill
 Heart
 Hemolymph
 Homeostasis
 Immune-related gene
 Innate immunity
 Mortality rate
 Nidovirales infection
 Nonhuman
 Penaeus monodon
 Priority journal
 Protein function
 Real time polymerase chain reaction
 RNA interference
 Tissue distribution
 Upregulation
 Yellow head virus
 Animal
 Blood cell
 Cardiac muscle
 Gene expression profiling
 Genetics
 Immunology
 Metabolism
 Penaeidae
 Physiology
 Roniviridae
 Virology
 Animals
 Arthropod Proteins
 Gene Expression Profiling
 Gene Silencing
 Gills
 Heart
 Hemocytes
 Myocardium
 Penaeidae
 Roniviridae
 Serine Peptidase Inhibitors, Kazal Type

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URI

https://hdl.handle.net/20.500.14740/3732

Collections

Scopus: Year 1983-2021

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