Publication: Stability indicating method to determine bioactive nucleosides in crude drugs, extracts, and products from cordyceps sinensis and cordyceps militaris
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Issued Date
2017
Resource Type
File Type
application/pdf
ISSN
1254685
Other identifier(s)
2-s2.0-85021907139
Rights Holder(s)
Scopus
Bibliographic Citation
Thai Journal of Pharmaceutical Sciences. Vol 41, No.2 (2017), p.52-60
Suggested Citation
Chutvirasakul B., Jongmeesuk W., Tirasomboonsiri P., Sansandee N., Tadtong S. Stability indicating method to determine bioactive nucleosides in crude drugs, extracts, and products from cordyceps sinensis and cordyceps militaris. Thai Journal of Pharmaceutical Sciences. Vol 41, No.2 (2017), p.52-60. Retrieved from: https://hdl.handle.net/20.500.14740/4723
Abstract
Objective: The aim of this study was to develop a stability indicating method to determine bioactive nucleosides including uridine, guanosine, adenosine, and cordycepin in crude drugs, extracts, and products from Cordyceps sinensis and Cordyceps militaris by reverse phase high performance liquid chromatography. Methods: The C8 column (250 mm × 4.6 mm; i.d. 5 µm) was used, and the mobile phase was a mixture of water (A) and acetonitrile (B). The system was: 0-15 min, 1% B; 15-30 min, 1-15% B. The flow rate was 1 mL/min and the injection volume was 10 µL with ultraviolet detection at 254 nm. Results: The correlation coefficients of linearity were more than 0.9995 for uridine (0.56-11.20 µg/mL), guanosine (0.56-11.21 µg/mL), adenosine (1.13-11.30 µg/mL), and cordycepin (0.279-2.793 µg/mL). The intra- and inter-day precisions were less than 2% and 3%, respectively. The accuracy of the method was in the range of 96.65-100.64%. The studied nucleosides were stable to heat at 90°C for 12 h but were more degraded in 0.1 N H2SO4 and 3% H2O2 than 0.1 N NaOH, and sunlight. Conclusion: The developed method was found to be specific to uridine, guanosine, adenosine, and cordycepin in the presence of sample matrices and their degradation products and could be applied to assess the stability of crude drugs, extracts, and products from C. sinensis and C. militaris. © 2017, Faculty of Pharmaceutical Sciences, Chulalongkorn University. All rights reserved.
Subject(s)
Acetonitrile
Adenosine
Cordycepin
Cordyceps militaris extract
Cordyceps sinensis extract
Guanosine
Hydrogen peroxide
Nucleoside
Sodium hydroxide
Sulfuric acid
Unclassified drug
Uridine
Accuracy
Analytic method
Article
Capillary electrophoresis
Controlled study
Cordyceps
Correlation coefficient
Drug stability
Drug structure
Flow rate
Heat
High performance liquid chromatography
Limit of detection
Limit of quantitation
Mass spectrometry
Nonhuman
Ophiocordyceps
Reversed phase high performance liquid chromatography
Sensitivity and specificity
Sunlight
Thermostability
Ultraviolet radiation
Adenosine
Cordycepin
Cordyceps militaris extract
Cordyceps sinensis extract
Guanosine
Hydrogen peroxide
Nucleoside
Sodium hydroxide
Sulfuric acid
Unclassified drug
Uridine
Accuracy
Analytic method
Article
Capillary electrophoresis
Controlled study
Cordyceps
Correlation coefficient
Drug stability
Drug structure
Flow rate
Heat
High performance liquid chromatography
Limit of detection
Limit of quantitation
Mass spectrometry
Nonhuman
Ophiocordyceps
Reversed phase high performance liquid chromatography
Sensitivity and specificity
Sunlight
Thermostability
Ultraviolet radiation