Publication: On-line single cell monitoring using electrochemical sensor coupled with droplet-based microfluidics
0
0
Issued Date
2015
Resource Type
File Type
application/pdf
Other identifier(s)
2-s2.0-84983372209
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. (2015), p.2056-2058
Suggested Citation
Suea-Ngam A., Rattanarat P., Siangproh W., Wongravee K., Chailapakul O., Srisa-Art M. On-line single cell monitoring using electrochemical sensor coupled with droplet-based microfluidics. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. (2015), p.2056-2058. Retrieved from: https://hdl.handle.net/20.500.14740/6205
Abstract
An electrochemical sensor coupled with droplet-based microfluidics was developed for on-line monitoring of single cells. In this work, the kinetics of glucose consumption of baker's yeast was monitored using a chip-based electrochemical sensor. The H2O2 product from the reaction between glucose oxidase and the excess glucose from the consumption of baker's yeast was detected using amperometric mesurements, in which the working electrode was a screen-printed carbon paste electrode (CPE) modified with silver nanoparticles (AgNPs) and polyaniline (PANI). Using this approach, monitoring of the kinetic reaction was be able to achieve at the single cell level. © 15CBMS-0001.
Subject(s)
Carbon
Drops
Electrodes
Glucose
Glucose oxidase
Glucose sensors
Microfluidics
Polyaniline
Silver
Yeast
Carbon paste electrode
Droplet-based microfluidics
Glucose consumption
Polyanilines (PAni)
Silver nanoparticles (AgNps)
Single-cell level
Singlecell analysis
Working electrode
Electrochemical sensors
Drops
Electrodes
Glucose
Glucose oxidase
Glucose sensors
Microfluidics
Polyaniline
Silver
Yeast
Carbon paste electrode
Droplet-based microfluidics
Glucose consumption
Polyanilines (PAni)
Silver nanoparticles (AgNps)
Single-cell level
Singlecell analysis
Working electrode
Electrochemical sensors