Publication:
Molecular classification of rumen fluke eggs in fecal specimens from Suphanburi Province, Thailand, based on cytochrome C oxidase subunit 1

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Issued Date

2020

Resource Type

Article

File Type

application/pdf

ISSN

24059390

DOI

10.1016/j.vprsr.2020.100382

Other identifier(s)

2-s2.0-85079396636

Rights Holder(s)

Scopus

Bibliographic Citation

Veterinary Parasitology: Regional Studies and Reports. Vol 20, (2020)

Suggested Citation

Anucherngchai S., Chontananarth T., Tejangkura T., Wongsawad C. Molecular classification of rumen fluke eggs in fecal specimens from Suphanburi Province, Thailand, based on cytochrome C oxidase subunit 1. Veterinary Parasitology: Regional Studies and Reports. Vol 20, (2020). doi:10.1016/j.vprsr.2020.100382 Retrieved from: https://hdl.handle.net/20.500.14740/4599

Title

Molecular classification of rumen fluke eggs in fecal specimens from Suphanburi Province, Thailand, based on cytochrome C oxidase subunit 1

Author(s)

Anucherngchai S.
 Chontananarth T.
 Tejangkura T.
 Wongsawad C.

Abstract

Rumen fluke infections have been known to cause paramphistomiasis in both wild and domestic animals worldwide. Occasionally, coinfections of rumen flukes (Carmyerius, Fischoederius, and Paramphistomum) with liver flukes (Fasciola) have been observed due to the similar life cycles that these two species share. This study involved an alternative approach that was developed to classify and distinguish rumen fluke eggs from other genera by using the polymerase chain reaction (PCR) method based on cytochrome c oxidase subunit 1 (COI). Thirty-eight fecal specimens of Bos taurus from Suphanburi Province, Central Thailand were examined using the formalin–ether sedimentation technique. PCR detection was then performed using COI-specific primers that were developed in this study. The results showed that this primer set can classify and distinguish the egg specimens into a separate clade of the genera comprising Gastrothylax, Carmyerius, Fischoederius, Paramphistomum, Explanatum, and Fasciola. Moreover, epidemiological mapping revealed coinfections of three genera of rumen flukes at some collection sites, leading to the need to further investigate Paramphistomoidea infection along with Fasciolidae infection within the endemic area. This data is important for monitoring the outbreak of these parasites in Suphanburi Province, Thailand. It can be applied for initiating surveillance programs of paramphistomiasis and fascioliasis in veterinary studies. © 2020 Elsevier B.V.

Subject(s)

Cytochrome c oxidase
 Cytochrome c oxidase subunit 1
 Unclassified drug
 Article
 Carmyerius
 Controlled study
 Endemic disease
 Epidemiological data
 Explanatum
 Fasciola
 Fecal egg count
 Feces analysis
 Fischoederius
 Gastrothylax
 Mixed infection
 Nonhuman
 Paramphistomum
 Polymerase chain reaction
 Rumen fluke
 Rumen fluke infection
 Taurine cattle
 Thailand
 Trematode
 Trematodiasis

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URI

https://hdl.handle.net/20.500.14740/4599

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Scopus: Year 1983-2021

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