Publication:
Detection of non-amplified Mycobacterium tuberculosis genomic DNA using piezoelectric DNA-based biosensors

1
0

Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

14248220

DOI

10.3390/s100301846

Other identifier(s)

2-s2.0-77955493608

Rights Holder(s)

Scopus

Bibliographic Citation

Sensors. Vol 10, No.3 (2010), p.1846-1858

Suggested Citation

Kaewphinit T., Santiwatanakul S., Promptmas C., Chansiri K. Detection of non-amplified Mycobacterium tuberculosis genomic DNA using piezoelectric DNA-based biosensors. Sensors. Vol 10, No.3 (2010), p.1846-1858. doi:10.3390/s100301846 Retrieved from: https://hdl.handle.net/20.500.14740/7602

Title

Detection of non-amplified Mycobacterium tuberculosis genomic DNA using piezoelectric DNA-based biosensors

Author(s)

Kaewphinit T.
 Santiwatanakul S.
 Promptmas C.
 Chansiri K.

Abstract

Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a selfassembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification. © 2010 by the authors.

Subject(s)

Cross hybridization
 DNA-based biosensors
 Genomic DNA
 Mycobacterium tuberculosis
 Oligonucleotide probes
 PCR amplification
 Piezoelectric biosensors
 Restriction enzymes
 Biosensors
 DNA
 Genes
 Oligonucleotides
 Piezoelectricity
 Quartz
 Polymerase chain reaction
 Bacterial DNA
 Gold
 Thiol derivative
 Article
 Chemistry
 Electrochemical analysis
 Electrode
 Genetic procedures
 Genetics
 Isolation and purification
 Metabolism
 Methodology
 Mycobacterium tuberculosis
 Oligonucleotide probe
 Biosensing Techniques
 DNA, Bacterial
 Electrochemical Techniques
 Electrodes
 Gold
 Mycobacterium tuberculosis
 Oligonucleotide Probes
 Sulfhydryl Compounds

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/7602

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By