Publication:
Effects of intramuscular administration of 1α,25(OH)2D3 during skeletal muscle regeneration on regenerative capacity, muscular fibrosis, and angiogenesis

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Issued Date

2016

Resource Type

Article

File Type

application/pdf

ISSN

87507587

DOI

10.1152/japplphysiol.01018.2015

Other identifier(s)

2-s2.0-84983604708

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Applied Physiology. Vol 120, No.12 (2016), p.1381-1393

Suggested Citation

Srikuea R., Hirunsai M. Effects of intramuscular administration of 1α,25(OH)2D3 during skeletal muscle regeneration on regenerative capacity, muscular fibrosis, and angiogenesis. Journal of Applied Physiology. Vol 120, No.12 (2016), p.1381-1393. doi:10.1152/japplphysiol.01018.2015 Retrieved from: https://hdl.handle.net/20.500.14740/5355

Title

Effects of intramuscular administration of 1α,25(OH)2D3 during skeletal muscle regeneration on regenerative capacity, muscular fibrosis, and angiogenesis

Author(s)

Srikuea R.
 Hirunsai M.

Abstract

The recent discovery of the vitaminDreceptor (VDR) in regenerating muscle raises the question regarding the action of Vitamin D3 on skeletal muscle regeneration. To investigate the action of Vitamin D3 on this process, the tibialis anterior muscle of male C57BL/6 mice (10 wk of age) was injected with 1.2% BaCl2 to induce extensive muscle injury. The bioactive form of Vitamin D3 [1α,25(OH)2D3] was administered daily via intramuscular injections during the regenerative phase (days 4-7 postinjury). Physiological and supraphysiological doses of 1α,25(OH)2D3 relative to 1 αg/kg muscle wet weight and mouse body weight were investigated. Muscle samples were collected on day 8 postinjury to examine proteins related to Vitamin D3 metabolism (VDR, CYP24A1, and CYP27B1), satellite cell differentiation and regenerative muscle fiber formation [myogenin and embryonic myosin heavy chain (EbMHC)], protein synthesis signaling (Akt, p70 S6K1, 4E-BP1, and myostatin), fiber-Type composition (fast and slow MHCs), fibrous formation (vimentin), and angiogenesis (CD31). Administration of 1α,25(OH)2D3 at physiological and supraphysiological doses enhanced VDR expression in regenerative muscle. Moreover, CYP24A1 and vimentin expression was increased, accompanying decreased myogenin and EbMHC expression at the supraphysiological dose. However, there was no change in CYP27B1, Akt, p70 S6K1, 4E-BP1, myostatin, fast and slow MHCs, or CD31 expression at any dose investigated. Taken together, administration of 1α,25(OH)2D3 at a supraphysiological dose decreased satellite cell differentiation, delayed regenerative muscle fiber formation, and increased muscular fibrosis. However, protein synthesis signaling, fiber-Type composition, and angiogenesis were not affected by either 1α,25(OH)2D3 administration at a physiological or supraphysiological dose. © 2016 the American Physiological Society.

Subject(s)

Calcitriol receptor
 Colecalciferol
 Myogenin
 Myosin heavy chain
 Animal
 C57BL mouse
 Cell differentiation
 Drug effects
 Fibrosis
 Intramuscular drug administration
 Male
 Metabolism
 Morphogenesis
 Mouse
 Muscle disease
 Neovascularization (pathology)
 Procedures
 Regeneration
 Skeletal muscle
 Wound healing
 Animals
 Cell Differentiation
 Cholecalciferol
 Fibrosis
 Injections, Intramuscular
 Male
 Mice
 Mice, Inbred C57BL
 Morphogenesis
 Muscle, Skeletal
 Muscular Diseases
 Myogenin
 Myosin Heavy Chains
 Neovascularization, Pathologic
 Receptors, Calcitriol
 Regeneration
 Wound Healing

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URI

https://hdl.handle.net/20.500.14740/5355

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Scopus: Year 1983-2021

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