Publication:
Differential diagnosis of human lymphatic filariasis using PCR-RFLP

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Issued Date

2000

Resource Type

Article

File Type

application/pdf

ISSN

8908508

DOI

10.1006/mcpr.1999.0283

Other identifier(s)

2-s2.0-0034067693

Rights Holder(s)

Scopus

Bibliographic Citation

Molecular and Cellular Probes. Vol 14, No.1 (2000), p.41-46

Suggested Citation

Thanomsub B.W., Chansiri K., Sarataphan N., Phantana S. Differential diagnosis of human lymphatic filariasis using PCR-RFLP. Molecular and Cellular Probes. Vol 14, No.1 (2000), p.41-46. doi:10.1006/mcpr.1999.0283 Retrieved from: https://hdl.handle.net/20.500.14740/7041

Title

Differential diagnosis of human lymphatic filariasis using PCR-RFLP

Author(s)

Thanomsub B.W.
 Chansiri K.
 Sarataphan N.
 Phantana S.

Abstract

Filariasis is still a public health problem in tropical countries. The most common causative agents of human filariasis are Wuchereria bancrofti and Brugia malayi. Traditional methods used to detect filarial parasites in human, animal and vector populations are tedious, time consuming, and confer little guarantee of sensitivity and species specificity. We have developed a rapid and specific method to detect filarial parasite DNAs in blood and mosquito samples using the polymerase chain reaction (PCR) technique. The primers used are MF/F and MF/R which amplify a 1.5 kb glutathione peroxidase gene of filarial worms. Using the restriction fragment length polymorphism (RFLP) technique, these PCR products will be further digested with restriction enzymes either HpaI, PstI, AluI or HinfI to differentiate the genus of filaria. This PCR-RFLP technique can be apply to use in diagnosis and to differentiate between species of filaria in humans the reservoir host and the mosquito vector in endemic areas. (C) 2000 Academic Press.

Subject(s)

Ethidium bromide
 Glutathione peroxidase
 Restriction endonuclease
 Article
 Blood analysis
 Brugia malayi
 Cat
 Controlled study
 Differential diagnosis
 DNA determination
 Filariasis
 Gene amplification
 Human
 Mosquito
 Nonhuman
 Polymerase chain reaction
 Priority journal
 Restriction fragment length polymorphism
 Wuchereria bancrofti
 Animalia
 Brugia malayi
 Felis catus
 Filaria
 Nematoda
 Wuchereria bancrofti

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URI

https://hdl.handle.net/20.500.14740/7041

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Scopus: Year 1983-2021

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