Publication:
Potential of recombinant 2-Cys peroxiredoxin protein as a vaccine for Fasciola gigantica infection

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Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

144894

DOI

10.1016/j.exppara.2018.09.005

Other identifier(s)

2-s2.0-85054808400

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Experimental Parasitology. Vol 194, (2018), p.16-23

Suggested Citation

Sangpairoj K., Apisawetakan S., Changklungmoa N., Kueakhai P., Chaichanasak P., Sobhon P., Chaithirayanon K. Potential of recombinant 2-Cys peroxiredoxin protein as a vaccine for Fasciola gigantica infection. Experimental Parasitology. Vol 194, (2018), p.16-23. doi:10.1016/j.exppara.2018.09.005 Retrieved from: https://hdl.handle.net/20.500.14740/5809

Title

Potential of recombinant 2-Cys peroxiredoxin protein as a vaccine for Fasciola gigantica infection

Author(s)

Sangpairoj K.
 Apisawetakan S.
 Changklungmoa N.
 Kueakhai P.
 Chaichanasak P.
 Sobhon P.
 Chaithirayanon K.

Abstract

Helminth 2-cys peroxiredoxin (Prx) is a major antioxidant enzyme that protects parasites against hydrogen peroxide-generating oxidative stress from the hosts’ immune responses. This enzyme has been found in all stages of the tropical liver fluke, Fasciola gigantica. To investigate the potential of the recombinant F. gigantica Prx-2 (rFgPrx-2) as a vaccine candidate, vaccine trials in mice were carried out. In this study, the ICR mice were immunized with rFgPrx-2 combined with Freund's adjuvant and infected with F. gigantica metacercariae. The vaccine efficacy was estimated by quantitate fluke recovery, antibody levels and liver function. The protection by rFgPrx-2 against F. gigantica infection was achieved at 43–46% compared with adjuvant-infected and non-immunized-infected control groups, respectively. The vaccine elicited both Th1 and Th2 humoral immune responses with predominance of Th2 as indicated by the higher level of IgG1 in sera of immunized mice. However, the levels of liver damage markers, serum glutamate oxalic transaminase (SGOT) and serum glutamic pyruvate transaminase (SGPT) in rFgPrx-2 immunized group did not show significant difference in comparison with the controls. This study suggested that rFgPrx-2 may have a potential as a vaccine against tropical fasciolosis. © 2018

Subject(s)

Alanine aminotransferase
 Antibody
 Aspartate aminotransferase
 Freund adjuvant
 Immunoglobulin G1
 Peroxiredoxin 2
 Alanine aminotransferase
 Aspartate aminotransferase
 Freund adjuvant
 Helminth antibody
 Immunoglobulin G
 Peroxiredoxin
 Recombinant protein
 Vaccine
 Alanine aminotransferase blood level
 Animal cell
 Animal experiment
 Animal model
 Animal tissue
 Antibody blood level
 Article
 Aspartate aminotransferase blood level
 Controlled study
 Drug efficacy
 Fasciola gigantica
 Fascioliasis
 Female
 Humoral immunity
 Infection prevention
 Liver function
 Liver injury
 Metacercaria
 Mouse
 Nonhuman
 Priority journal
 Th1 cell
 Th2 cell
 Animal
 Blood
 Enzyme linked immunosorbent assay
 Enzymology
 Fasciola
 Fascioliasis
 Immunology
 Institute for Cancer Research mouse
 Liver
 Lymnaea
 Parasitology
 Pathology
 Physiology
 Randomization
 Alanine Transaminase
 Animals
 Antibodies, Helminth
 Aspartate Aminotransferases
 Enzyme-Linked Immunosorbent Assay
 Fasciola
 Fascioliasis
 Female
 Freund's Adjuvant
 Immunoglobulin G
 Liver
 Lymnaea
 Mice
 Mice, Inbred ICR
 Peroxiredoxins
 Random Allocation
 Recombinant Proteins
 Vaccines

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URI

https://hdl.handle.net/20.500.14740/5809

Collections

Scopus: Year 1983-2021

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