Publication:
Molecular isolation and characterization of translationally controlled tumor protein (TCTP) gene from Macrobrachium rosenbergii

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Issued Date

2020

Resource Type

Article

File Type

application/pdf

ISSN

9676120

DOI

10.1007/s10499-020-00571-8

Other identifier(s)

2-s2.0-85089175354

Rights

Srinakharinwirot University

Rights Holder(s)

Scopus

Bibliographic Citation

Aquaculture International. Vol 28, No.6 (2020), p.2173-2190

Suggested Citation

Pasookhush P., Vaniksampanna A., Sithigorngul P., Longyant S., Chaivisuthangkura P. Molecular isolation and characterization of translationally controlled tumor protein (TCTP) gene from Macrobrachium rosenbergii. Aquaculture International. Vol 28, No.6 (2020), p.2173-2190. doi:10.1007/s10499-020-00571-8 Retrieved from: https://hdl.handle.net/20.500.14740/5262

Title

Molecular isolation and characterization of translationally controlled tumor protein (TCTP) gene from Macrobrachium rosenbergii

Author(s)

Pasookhush P.
 Vaniksampanna A.
 Sithigorngul P.
 Longyant S.
 Chaivisuthangkura P.

Abstract

Translationally controlled tumor protein (TCTP) or fortilin is a highly conserved protein among eukaryotes and has important biological roles including anti-apoptotic activity which involves the elimination of virus-infected cells. In this study, the full-length cDNA of Macrobrachium rosenbergii TCTP (MrTCTP) was isolated. The cDNA sequence consisted of 783 bp with a 507-bp open reading frame encoding 168 amino acids. MrTCTP demonstrated all major characteristics of TCTP which include (1) highly homologous to TCTP protein family, (2) carrying two TCTP signatures, and (3) contained highly conserved amino acid sequences. The predicted structure of MrTCTP also revealed typical structure of TCTP which had three major domains including ß-stranded core domain, helical domain, and flexible loop. The predicted molecular mass of MrTCTP was 18.94 kDa and sequence displays 83.93 and 78.57% identity to that of Eriocheir sinensis TCTP and Fenneropenaeus chinensis TCTP, respectively. Phylogenetic analysis revealed that the deduced amino acid sequence of MrTCTP was clustered into the invertebrate branch along with the sequences from arthropods and crustaceans. The mRNA expression showed that MrTCTP expressed in every tissue examined, preferentially in hepatopancreas and muscle. In addition, MrTCTP expression in the muscle was upregulated at 1 to 4 days after infection with M. rosenbergii nodavirus (MrNV). Finally, silencing of MrTCTP increased mortality rates post-MrNV challenge. Taken together, the study shows that MrTCTP may play an important role in shrimp immune response against MrNV infection. © 2020, Springer Nature Switzerland AG.

Subject(s)

Apoptosis
 DNA
 Eukaryote
 Gene
 Genetic analysis
 Genetic structure
 Immune response
 Isolation effect
 Molecular analysis
 Protein
 RNA
 Shrimp culture
 Tumor
 Viral disease
 Arthropoda
 Crustacea
 Decapoda (Crustacea)
 Eriocheir sinensis
 Eukaryota
 Fenneropenaeus chinensis
 Invertebrata
 Macrobrachium rosenbergii
 Macrobrachium rosenbergii nodavirus

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URI

https://hdl.handle.net/20.500.14740/5262

Collections

Scopus: Year 1983-2021

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