Publication:
Holothuria scabra Extract Induces Cell Apoptosis and Suppresses Warburg Effect by Down-Regulating Akt/mTOR/HIF-1 Axis in MDA-MB-231 Breast Cancer Cells

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Issued Date

2021

Resource Type

Article

File Type

application/pdf

ISSN

1635581

DOI

10.1080/01635581.2020.1814825

Other identifier(s)

2-s2.0-85090147639

Rights Holder(s)

Scopus

Bibliographic Citation

Nutrition and Cancer. Vol 73, No.10 (2021), p.1964-1975

Suggested Citation

Yurasakpong L., Apisawetakan S., Pranweerapaiboon K., Sobhon P., Chaithirayanon K. Holothuria scabra Extract Induces Cell Apoptosis and Suppresses Warburg Effect by Down-Regulating Akt/mTOR/HIF-1 Axis in MDA-MB-231 Breast Cancer Cells. Nutrition and Cancer. Vol 73, No.10 (2021), p.1964-1975. doi:10.1080/01635581.2020.1814825 Retrieved from: https://hdl.handle.net/20.500.14740/8126

Title

Holothuria scabra Extract Induces Cell Apoptosis and Suppresses Warburg Effect by Down-Regulating Akt/mTOR/HIF-1 Axis in MDA-MB-231 Breast Cancer Cells

Author(s)

Yurasakpong L.
 Apisawetakan S.
 Pranweerapaiboon K.
 Sobhon P.
 Chaithirayanon K.

Abstract

Introduction: Cancer cells utilize the modified glucose metabolism known as Warburg effect, with lactate production as the end product. In the search for alternative therapy, the body wall of sea cucumbers contains various substances with pharmacological activities. Herein, we investigate the effect of Holothuria scabra extract on the viability and Warburg effect of aggressive breast cancer cells. Methods: Body wall of H. scabra was extracted using 95% ethanol. Triple-negative breast cancer cells, MDA-MB-231, were treated with the extract at various concentrations under normoglycemic and hyperglycemic conditions. Cytotoxicity test was performed using MTT assay. Apoptotic proteins were quantified using Western blot. Apoptotic cells were stained with Hoechst 33342. Lactate production was determined using L-lactate assay kit. Results: By MTT assay, H. scabra extract suppressed the viability of breast cancer cells in a dose-dependent and time-dependent manner by enhancing apoptosis, indicated by a marked increase of proapoptotic Bax and pro-caspase three expressions, and decreased expression of anti-apoptotic Bcl-2. The extract could reduce hexokinase II expression, leading to reduced lactate production by blocking the Akt/mTOR/HIF-1 axis. Discussion: Overall findings indicated that H. scabra extract could be a possible therapeutic against breast cancer progression in patients with hyperglycemia, for instance, diabetes mellitus. © 2020 Taylor & Francis Group, LLC.

Subject(s)

Actin
 Alcohol
 Apoptosis inducing factor
 Caspase 3
 Flavanone derivative
 Glucose
 Glycolytic enzyme
 Hexokinase
 Hexokinase 1
 Hexokinase 2
 Hoe 33342
 Holothuria scabra extract
 Hypoxia inducible factor 1alpha
 Lactic acid
 Mammalian target of rapamycin
 Plant extract
 Protein Bax
 Protein bcl 2
 Protein kinase B
 Triterpenoid
 Unclassified drug
 MTOR protein, human
 Plant extract
 Protein kinase B
 Target of rapamycin kinase
 Akt/mTOR signaling
 Antineoplastic activity
 Apoptosis
 Article
 Cell viability
 Chemical composition
 Chromatin condensation
 Concentration response
 Controlled study
 Down regulation
 Drug cytotoxicity
 Drug isolation
 Drug mechanism
 Glycolysis
 Holothuria
 Holothuria scabra
 Human
 Human cell
 Hyperglycemia
 IC50
 MDA-MB-231 cell line
 MTT assay
 Protein expression
 Skin fibroblast
 Triple negative breast cancer
 Upregulation
 Warburg effect
 Western blotting
 Animal
 Apoptosis
 Breast tumor
 Cell proliferation
 Female
 Genetics
 Triple negative breast cancer
 Tumor cell line
 Animals
 Apoptosis
 Breast Neoplasms
 Cell Line, Tumor
 Cell Proliferation
 Female
 Holothuria
 Humans
 Plant Extracts
 Proto-Oncogene Proteins c-akt
 TOR Serine-Threonine Kinases
 Triple Negative Breast Neoplasms

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URI

https://hdl.handle.net/20.500.14740/8126

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Scopus: Year 1983-2021

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