Publication:
Electrochemical paper-based peptide nucleic acid biosensor for detecting human papillomavirus

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Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

32670

DOI

10.1016/j.aca.2016.11.071

Other identifier(s)

2-s2.0-85006381853

Rights Holder(s)

Scopus

Bibliographic Citation

Analytica Chimica Acta. Vol 952, (2017), p.32-40

Suggested Citation

Teengam P., Siangproh W., Tuantranont A., Henry C.S., Vilaivan T., Chailapakul O. Electrochemical paper-based peptide nucleic acid biosensor for detecting human papillomavirus. Analytica Chimica Acta. Vol 952, (2017), p.32-40. doi:10.1016/j.aca.2016.11.071 Retrieved from: https://hdl.handle.net/20.500.14740/4267

Title

Electrochemical paper-based peptide nucleic acid biosensor for detecting human papillomavirus

Author(s)

Teengam P.
 Siangproh W.
 Tuantranont A.
 Henry C.S.
 Vilaivan T.
 Chailapakul O.

Abstract

A novel paper-based electrochemical biosensor was developed using an anthraquinone-labeled pyrrolidinyl peptide nucleic acid (acpcPNA) probe (AQ-PNA) and graphene-polyaniline (G-PANI) modified electrode to detect human papillomavirus (HPV). An inkjet printing technique was employed to prepare the paper-based G-PANI-modified working electrode. The AQ-PNA probe baring a negatively charged amino acid at the N-terminus was immobilized onto the electrode surface through electrostatic attraction. Electrochemical impedance spectroscopy (EIS) was used to verify the AQ-PNA immobilization. The paper-based electrochemical DNA biosensor was used to detect a synthetic 14-base oligonucleotide target with a sequence corresponding to human papillomavirus (HPV) type 16 DNA by measuring the electrochemical signal response of the AQ label using square-wave voltammetry before and after hybridization. It was determined that the current signal significantly decreased after the addition of target DNA. This phenomenon is explained by the rigidity of PNA-DNA duplexes, which obstructs the accessibility of electron transfer from the AQ label to the electrode surface. Under optimal conditions, the detection limit of HPV type 16 DNA was found to be 2.3 nM with a linear range of 10–200 nM. The performance of this biosensor on real DNA samples was tested with the detection of PCR-amplified DNA samples from the SiHa cell line. The new method employs an inexpensive and disposable device, which easily incinerated after use and is promising for the screening and monitoring of the amount of HPV-DNA type 16 to identify the primary stages of cervical cancer. © 2016 Elsevier B.V.

Subject(s)

Biosensors
 Cell culture
 Chemical detection
 DNA
 Electrochemical impedance spectroscopy
 Graphene
 Ink jet printing
 Ketones
 Nucleic acids
 Oligonucleotides
 Peptides
 Polyaniline
 Polymerase chain reaction
 Probes
 Voltammetry
 AcpcPNA
 Electrochemical biosensor
 ELectrochemical detection
 Electrochemical DNA biosensors
 Electrochemical signals
 Electrostatic attractions
 Human papillomavirus
 Square wave voltammetry
 Electrochemical electrodes
 Anthraquinone
 Graphene
 Nucleic acid
 Polyaniline
 Pyrrolidine derivative
 Pyrrolidinyl peptide nucleic acid
 Unclassified drug
 Peptide nucleic acid
 Virus DNA
 Amino terminal sequence
 Article
 Biosensor
 Controlled study
 Electrochemical detection
 Electrochemical impedance spectroscopy
 Electron transport
 Human papillomavirus type 16
 Hybridization
 Limit of detection
 Limit of quantitation
 Nonhuman
 Paper based electrochemical biosensor
 Polymerase chain reaction
 Potentiometry
 Priority journal
 Reproducibility
 Square wave voltammetry
 Static electricity
 Uterine cervix cancer
 Virus detection
 Chemistry
 Electrochemical analysis
 Electrode
 Genetic procedures
 Human
 Human papillomavirus type 16
 Isolation and purification
 Nucleic acid hybridization
 Tumor cell line
 Electrodes
 Ink Jet Printing
 Nucleic Acids
 Peptides
 Biosensing Techniques
 Cell Line, Tumor
 DNA, Viral
 Electrochemical Techniques
 Electrodes
 Human papillomavirus 16
 Humans
 Nucleic Acid Hybridization
 Peptide Nucleic Acids

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URI

https://hdl.handle.net/20.500.14740/4267

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Scopus: Year 1983-2021

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