Publication:
Squamous carcinoma cells influence monocyte phenotype and suppress lipopolysaccharide-induced tnf-alpha in monocytes

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

3603997

DOI

10.1007/s10753-009-9175-6

Other identifier(s)

2-s2.0-77956187379

Rights Holder(s)

Scopus

Bibliographic Citation

Inflammation. Vol 33, No.4 (2010), p.207-223

Suggested Citation

Lam-Ubol A., Hopkin D., Letuchy E.M., Kurago Z.B. Squamous carcinoma cells influence monocyte phenotype and suppress lipopolysaccharide-induced tnf-alpha in monocytes. Inflammation. Vol 33, No.4 (2010), p.207-223. doi:10.1007/s10753-009-9175-6 Retrieved from: https://hdl.handle.net/20.500.14740/7558

Title

Squamous carcinoma cells influence monocyte phenotype and suppress lipopolysaccharide-induced tnf-alpha in monocytes

Author(s)

Lam-Ubol A.
 Hopkin D.
 Letuchy E.M.
 Kurago Z.B.

Abstract

Bacteria and chronic inflammation are present in squamous cell carcinoma of the head and neck (HNSCC), but their roles in the pathogenesis of HNSCC are unclear. Our studies described here revealed that human monocytes co-cultured short term with HNSCC cells were more likely to express CD16, and CD16 + small mononuclear cells were common in HNSCC specimens. In addition, we identified monocytes as the primary source of LPS-induced IL-6 and TNF-alpha in the monocyte-HNSCC co-cultures. Remarkably, relative to LPS-stimulated monocytes cultured alone, HNSCC cells profoundly suppressed LPS-induced TNF-alpha in monocytes, without compromising IL-6 production. High levels of cytoprotective factors like IL-6 and low levels of TNF-alpha are important for the tumor microenvironment that enables tumor cell survival, affects monocyte differentiation and may contribute to tumor colonization by bacteria. This study provides novel observations that HNSCC cells affect monocyte phenotype and function, which are relevant to the regulation of the HNSCC microenvironment.© 2010 Springer Science+Business Media, LLC.

Subject(s)

CD16 antigen
 CD56 antigen
 Interleukin 6
 Lipopolysaccharide
 Tumor necrosis factor alpha
 Fc receptor
 FCGR3B protein, human
 Glycosylphosphatidylinositol anchored protein
 IL6 protein, human
 Interleukin 6
 Lipopolysaccharide
 Lipopolysaccharide, E. coli 026-B6
 Tumor necrosis factor alpha
 Antigen expression
 Article
 Cancer cell
 Cell differentiation
 Cell protection
 Cell survival
 Controlled study
 Cytokine production
 Cytokine release
 Head and neck carcinoma
 Human
 Human cell
 Keratinocyte
 Monocyte
 Mouth carcinoma
 Pathogenesis
 Phenotype
 Regulatory mechanism
 Squamous cell carcinoma
 Tumor microenvironment
 Upregulation
 Biosynthesis
 Cell culture
 Coculture
 Cytology
 Disease course
 Drug effects
 Head and neck tumor
 Immunology
 Inflammation
 Monocyte
 Mouth mucosa
 Pathology
 Phagocytosis
 Phenotype
 Secretion (process)
 Squamous cell carcinoma
 Stomatitis
 Tumor cell line
 Carcinoma, Squamous Cell
 Cell Line, Tumor
 Cells, Cultured
 Coculture Techniques
 Disease Progression
 Head and Neck Neoplasms
 Humans
 Inflammation
 Interleukin-6
 Keratinocytes
 Lipopolysaccharides
 Monocytes
 Mouth Mucosa
 Phagocytosis
 Phenotype
 Receptors, IgG
 Stomatitis
 Tumor Necrosis Factor-alpha
 Carcinoma, Squamous Cell
 Cell Line, Tumor
 Cells, Cultured
 Coculture Techniques
 Disease Progression
 GPI-Linked Proteins
 Head and Neck Neoplasms
 Humans
 Inflammation
 Interleukin-6
 Keratinocytes
 Lipopolysaccharides
 Monocytes
 Mouth Mucosa
 Phagocytosis
 Phenotype
 Receptors, IgG
 Stomatitis
 Tumor Necrosis Factor-alpha

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URI

https://hdl.handle.net/20.500.14740/7558

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Scopus: Year 1983-2021

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