Publication: Characterization of a novel Type-I Crustin (carcininPm2) from black tiger shrimp Penaeus monodon
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Issued Date
2024-05-01
Resource Type
ISSN
10504648
eISSN
10959947
Scopus ID
2-s2.0-85189165052
Pubmed ID
38513915
Journal Title
Fish and Shellfish Immunology
Volume
148
Rights Holder(s)
SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology Vol.148 (2024)
Suggested Citation
Donpudsa S., Piaprad O., Tassanakajon A., Rimphanitchayakit V., Visetnan S. Characterization of a novel Type-I Crustin (carcininPm2) from black tiger shrimp Penaeus monodon. Fish and Shellfish Immunology Vol.148 (2024). doi:10.1016/j.fsi.2024.109520 Retrieved from: https://hdl.handle.net/20.500.14740/20459
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Abstract
Carcinins are type-I crustins from crustaceans and play an important role in innate immune system. In this study, type-I crustins, carcininPm1 and carcininPm2, from the hemocytes of Penaeus monodon were identified. Comparison of their amino acid sequences and the phylogenetic tree revealed that they were closely related to the other crustacean carcinin proteins, but were clustered into different groups of the carcinin proteins. The full-length amino acids of carcininPm1 and carcininPm2 were 92 and 111 residues, respectively. CarcininPm1 and carcininPm2 were expressed mainly in hemocytes and intestine compared to the other tissues. The expression of carcininPm1 and carcininPm2 were dramatically increased in early time of bacterial challenged shrimp hemocytes. In contrast, the carcininPm1 and carcininPm2 were expressed in response to late state of YHV-infected shrimp hemocytes where the copy number of virus was high. The recombinant carcininPm2 (rcarcininPm2) but not its WAP domain (rcarcininPm2_WAP) exhibited antimicrobial activity against Vibrio harveyi and Vibrio parahaemolyticus AHPND but not other bacteria tested. The rcarcininPm2 was able to prolong the survival rate of VH-treated post larval shrimp from about 102 h to 156 h. These studies indicated that the carcininPm2 possessed the potential and challenges as antibacterial in innate immunity of shrimp.