Publication: Targeted gold nanohybrids functionalized with folate-hydrophobic-quaternized pullulan delivering camptothecin for enhancing hydrophobic anticancer drug efficacy
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Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
ISSN
20734360
Other identifier(s)
2-s2.0-85112697880
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Polymers. Vol 13, No.16 (2021)
Suggested Citation
Laksee S., Supachettapun C., Muangsin N., Lertsarawut P., Rattanawongwiboon T., Sricharoen P., Limchoowong N., Chutimasakul T., Kwamman T., Hemvichian K. Targeted gold nanohybrids functionalized with folate-hydrophobic-quaternized pullulan delivering camptothecin for enhancing hydrophobic anticancer drug efficacy. Polymers. Vol 13, No.16 (2021). doi:10.3390/polym13162670 Retrieved from: https://hdl.handle.net/20.500.14740/4779
Abstract
This study presented a green, facile and efficient approach for a new combination of targeted gold nanohybrids functionalized with folate-hydrophobic-quaternized pullulan delivering hydrophobic camptothecin (CPT-GNHs@FHQ-PUL) to enhance the efficacy, selectivity, and safety of these systems. New formulations of spherical CPT-GNHs@FHQ-PUL obtained by bio-inspired strategy were fully characterized by TEM, EDS, DLS, zeta-potential, UV-vis, XRD, and ATR-FTIR anal-yses, showing a homogeneous particles size with an average size of approximately 10.97 ± 2.29 nm. CPT was successfully loaded on multifunctional GNHs@FHQ-PUL via intermolecular interactions. Moreover, pH-responsive CPT release from newly formulated-CPT-GNHs@FHQ-PUL exhibited a faster release rate under acidic conditions. The intelligent CPT-GNHs@FHQ-PUL (IC50 = 6.2 µM) displayed a 2.82-time higher cytotoxicity against human lung cancer cells (Chago-k1) than CPT alone (IC50 = 2.2 µM), while simultaneously exhibiting less toxicity toward normal human lung cells (Wi-38). These systems also showed specific uptake by folate receptor-mediated endocytosis, exhibited excellent anticancer activity, induced the death of cells by increasing apoptosis pathway (13.97%), and arrested the cell cycle at the G0-G1 phase. The results of this study showed that the delivery of CPT by smart GNHs@FHQ-PUL systems proved to be a promising strategy for increasing its chemotherapeutic effects. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subject(s)
Biological organs
Biomimetics
Cell death
Controlled drug delivery
Cytotoxicity
Gold
Hydrophobicity
Molecular biology
Nanostructured materials
Respiratory system
Acidic conditions
Anticancer activities
Apoptosis pathways
Camptothecin (CPT)
Folate receptor
Human lung cancer cells
Human lung cells
Intermolecular interactions
Targeted drug delivery
Biomimetics
Cell death
Controlled drug delivery
Cytotoxicity
Gold
Hydrophobicity
Molecular biology
Nanostructured materials
Respiratory system
Acidic conditions
Anticancer activities
Apoptosis pathways
Camptothecin (CPT)
Folate receptor
Human lung cancer cells
Human lung cells
Intermolecular interactions
Targeted drug delivery