Publication:
Scanning electrochemical microscopy of model neurons: Imaging and real-time detection of morphological changes

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Issued Date

2003

Resource Type

Article

File Type

application/pdf

ISSN

32700

DOI

10.1021/ac026166v

Other identifier(s)

2-s2.0-0037312636

Rights Holder(s)

Scopus

Bibliographic Citation

Analytical Chemistry. Vol 75, No.3 (2003), p.563-571

Suggested Citation

Liebetrau J.M., Miller H.M., Baur J.E., Takacs S.A., Anupunpisit V., Garris P.A., Wipf D.O. Scanning electrochemical microscopy of model neurons: Imaging and real-time detection of morphological changes. Analytical Chemistry. Vol 75, No.3 (2003), p.563-571. doi:10.1021/ac026166v Retrieved from: https://hdl.handle.net/20.500.14740/6593

Title

Scanning electrochemical microscopy of model neurons: Imaging and real-time detection of morphological changes

Author(s)

Liebetrau J.M.
 Miller H.M.
 Baur J.E.
 Takacs S.A.
 Anupunpisit V.
 Garris P.A.
 Wipf D.O.

Abstract

Living PC12 cells, a model cell type for studying neuronal function, were imaged using the negative feedback mode of a scanning electrochemical microscope (SECM). Six biocompatible redox mediators were successfully identified from a large pool of candidates and were then used for imaging PC12 cells before and after exposure to nerve growth factor (NGF). When exposed to NGF, cells differentiate into a neuron phenotype by growing narrow neurites (1-2 μm wide) that can extend >100 μm from the cell proper. We demonstrate that carbon fiber electrodes with reduced tip diameters can be used for imaging both the cell proper and these neurites. Regions of decreased current, possibly resulting from raised features not identifiable by light microscopy, are clearly evident in the SECM images. Changes in the morphology of undifferentiated PC12 cells could be detected in real time with the SECM. After exposure to hypotonic and hypertonic solutions, reversible changes in cell height of <2 μm were measured.

Subject(s)

Biocompatibility
 Carbon fibers
 Cells
 Imaging techniques
 Microscopic examination
 Morphology
 Neurology
 Scanning electrochemical microscopy (SECM)
 Electrochemistry
 Dopamine
 Animal cell
 Article
 Cell strain
 Cell structure
 Cell type
 Cyclic potentiometry
 Dopamine release
 Electrochemical analysis
 Electrode
 High performance liquid chromatography
 Microscopy
 Model
 Morphology
 Nerve cell
 Nerve cell differentiation
 Neurotransmitter release
 Nonhuman
 Rat
 Scanning electrochemical microscopy
 Scanning electron microscopy
 Animals
 Diagnostic Imaging
 Electrochemistry
 Microelectrodes
 Microscopy, Electron, Scanning
 Nerve Growth Factor
 Neurons
 PC12 Cells
 Rats

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URI

https://hdl.handle.net/20.500.14740/6593

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Scopus: Year 1983-2021

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