Publication: Molecular detection of three intestinal cestode species (Raillietina echinobothrida, R. tetragona, R. cesticillus) from poultry in Thailand
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Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
ISSN
3079457
Other identifier(s)
2-s2.0-85114366159
Rights Holder(s)
Scopus
Bibliographic Citation
Avian Pathology. Vol 50, No.4 (2021), p.321-326
Suggested Citation
Panich W., Chontananarth T. Molecular detection of three intestinal cestode species (Raillietina echinobothrida, R. tetragona, R. cesticillus) from poultry in Thailand. Avian Pathology. Vol 50, No.4 (2021), p.321-326. doi:10.1080/03079457.2021.1926920 Retrieved from: https://hdl.handle.net/20.500.14740/7987
Author(s)
Abstract
Cestodes belonging to the genus Raillietina are a major veterinary health problem affecting the poultry industry, particularly chickens (Gallus gallus domesticus) and ducks (Anas playtrhynchos domesticus). The traditional method for accurately detecting this cestode based on their morphological characteristics is rather difficult due to the large number of morphological similarities. Consequently, this study aimed to develop specific primers for R. echinobothrida, R. tetragona, and R. cesticillus detection that could be used to indicate epidemic areas for protection and infection control. Specific primers were manually designed based on the internal transcribed spacer 2 region and validated, establishing the optimal temperature, final concentration in PCR mixture, specificity, and sensitivity of each primer set. The results showed that the primers amplify specific species without cross-amplifying other parasites and hosts. The PCR products were about 473, 352, and 397 bp long for R. echinobothrida, R. tetragona, and R. cesticillus, respectively. The sensitivity test demonstrated that R. echinobothrida and R. cesticillus-specific primers detect a minimum of 5×10−2 ng DNA, while R. tetragona-specific primers detect a minimum of 0.5 ng genomic DNA. The specific primers successfully developed in this study might be useful for detecting cysticercoids in intermediate hosts or adult stages in poultry for epidemiological surveys, management and control of infection. RESEARCH HIGHLIGHTS This study established specific primers for Raillietina species detection. The ITS2 region is an effective molecular marker for Raillietina identification. © 2021 Houghton Trust Ltd.
Subject(s)
Genomic DNA
Internal transcribed spacer 2
Adult
Article
Cestode
Cestodiasis
Chicken
Concentration (parameter)
Controlled study
Cysticercoid
Diagnostic accuracy
Duck
Epidemic
Infection control
Intermediate host
Molecular diagnosis
Nonhuman
Parasite identification
Polymerase chain reaction
Poultry
Raillietina cesticillus
Raillietina echinobothrida
Raillietina tetragona
Sensitivity and specificity
Species difference
Thailand
Internal transcribed spacer 2
Adult
Article
Cestode
Cestodiasis
Chicken
Concentration (parameter)
Controlled study
Cysticercoid
Diagnostic accuracy
Duck
Epidemic
Infection control
Intermediate host
Molecular diagnosis
Nonhuman
Parasite identification
Polymerase chain reaction
Poultry
Raillietina cesticillus
Raillietina echinobothrida
Raillietina tetragona
Sensitivity and specificity
Species difference
Thailand