Publication:
Isoflavone genistein modulates the protein expression of toll-like receptors in cancerous human endometrial cells

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Issued Date

2015

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84957681300

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 98, (2015), p.S31-S38

Suggested Citation

Buathong N., Poonyachoti S., Deachapunya C. Isoflavone genistein modulates the protein expression of toll-like receptors in cancerous human endometrial cells. Journal of the Medical Association of Thailand. Vol 98, (2015), p.S31-S38. Retrieved from: https://hdl.handle.net/20.500.14740/6072

Title

Isoflavone genistein modulates the protein expression of toll-like receptors in cancerous human endometrial cells

Author(s)

Buathong N.
 Poonyachoti S.
 Deachapunya C.

Abstract

Objective: The present study aimed to investigate whether genistein, a potent phytoestrogen mainly found in soybean, modulated the expression of TLRs 2, 3, 4 and 9 proteins in human endometrial epithelial cell line RL95-2 under basal and polyinosinic-polycytidylic acid (poly I: C) stimulated conditions to mimic viral infection. The genistein effects were also compared with 17β-estradiol. Material and Method: The RL95-2 cells were cultured in the estrogen-deprived media with or without poly I: C 30 min prior to incubation with genistein (10-7, 10-6 or 10-5 M) or 17β-estradiol (10-9 M) for 48 h. The TLRs protein expression was analyzed by semi-quantitative Western blot. Results: The cells expressed TLRs 3, 4 and 9 but a very low level of TLR2 proteins. Poly I: C significantly increased the TLRs 2 and 9 protein expressions whereas the TLRs 3 and 4 were reduced. Under basal condition, genistein at 10-7 M increased the TLR2 while 17β-estradiol decreased the TLR4. All concentrations of genistein and 17β-estradiol attenuated the poly I: C induced increase in the TLR2. By contrast, both genistein at 10-5 M and 17β-estradiol further potentiated the TLR4 suppressed by poly I: C. Only 17β-estradiol was found to antagonize the poly I: C-induced changes in TLRs 3 and 9. Conclusion: Taken together, the present results that genistein increased the basal TLR2 and attenuated the viral componentinduced TLR2 protein expression in human endometrial epithelial cells may indicate the potential role of this soy isoflavone in promoting the uterine immune function and probably alleviating the inflammation of endometrium following pathogen invasion. © 2015, Medical Association of Thailand. All rights reserved.

Subject(s)

Dimethyl sulfoxide
 Estradiol
 Genistein
 Isoflavone derivative
 Phytoestrogen
 Toll like receptor
 Toll like receptor 2
 Toll like receptor 3
 Toll like receptor 4
 Toll like receptor 9
 Estradiol
 Genistein
 Phytoestrogen
 Article
 Controlled study
 Densitometry
 Double-stranded RNA virus
 Endometrial cancer cell line
 Endometrium carcinoma
 Human
 Human cell
 Innate immunity
 Polyacrylamide gel electrophoresis
 Protein expression
 Virus infection
 Western blotting
 Cell line
 Comparative study
 Drug effects
 Endometrium
 Endometrium tumor
 Epithelium cell
 Female
 Metabolism
 Blotting, Western
 Cell Line
 Endometrial Neoplasms
 Endometrium
 Epithelial Cells
 Estradiol
 Female
 Genistein
 Humans
 Phytoestrogens

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URI

https://hdl.handle.net/20.500.14740/6072

Collections

Scopus: Year 1983-2021

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