Publication: Comparison of acid-, alkaline-, and ionic liquid–treated Napier grass as an immobilization carrier for butanol production by Clostridium beijerinckii JCM 8026
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Issued Date
2020
Resource Type
File Type
application/pdf
ISSN
21906815
Other identifier(s)
2-s2.0-85096630376
Rights
Srinakharinwirot University
Rights Holder(s)
Scopus
Bibliographic Citation
Biomass Conversion and Biorefinery. Vol 10, No.4 (2020), p.1071-1082
Suggested Citation
Chinwatpaiboon P., Doolayagovit I., Boonsombuti A., Savarajara A., Luengnaruemitchai A. Comparison of acid-, alkaline-, and ionic liquid–treated Napier grass as an immobilization carrier for butanol production by Clostridium beijerinckii JCM 8026. Biomass Conversion and Biorefinery. Vol 10, No.4 (2020), p.1071-1082. doi:10.1007/s13399-019-00491-5 Retrieved from: https://hdl.handle.net/20.500.14740/5530
Abstract
Immobilization is a simple technique in which microorganisms adhere and agglomerate onto the porous material’s surface, resulting in a higher cell density, cell tolerance, and productivity. Acetone-butanol-ethanol (ABE) fermentation using immobilized Clostridium beijerinckii JCM 8026 on Napier grass (Pennisetum purpureum) chemically pretreated with H2SO4, NaOH, and 1-ethyl-3-methylimidazolium acetate (EMIM-OAc) was compared in order to investigate the effect of the treatment on butanol production. X-ray diffraction, Fourier-transform infrared spectroscopy, and surface area measurement analyses indicated that the alkaline-pretreated Napier grass sample had the highest crystalline level with the lowest lignin content (lignin/cellulose) and a high surface area compared with other pretreated samples, suggesting its potential as an immobilized material. The fermentation was operated in a batch system for 5 days with an initial glucose level of 60 g/L. Clostridium beijerinckii JCM 8026 immobilized on the NaOH-treated Napier grass gave the highest butanol concentration (8.99 g/L), which corresponded to a 24.7% and 25.6% higher concentration than that when the cells were immobilized on untreated Napier grass and free cell culture, respectively. It is likely that immobilization on NaOH-treated Napier grass increased the cells’ protection from environmental stresses and prevented their washing out due to its swollen structure within an enlarged surface area. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
Subject(s)
Acetone
Butenes
Cells
Clostridium
Fermentation
Fourier transform infrared spectroscopy
Ionic liquids
Lignin
Nanocrystalline materials
Porous materials
Radioactive waste vitrification
Sodium hydroxide
1-ethyl-3-methylimidazolium acetates
Abe fermentations
Acetone-butanol-ethanol fermentation
Clostridium beijerinckii
Immobilization carriers
Napier grass
Pre-Treatment
Surface area measurement
Cell immobilization
Acetone
Butenes
Cells
Fermentation
Porous Materials
Butenes
Cells
Clostridium
Fermentation
Fourier transform infrared spectroscopy
Ionic liquids
Lignin
Nanocrystalline materials
Porous materials
Radioactive waste vitrification
Sodium hydroxide
1-ethyl-3-methylimidazolium acetates
Abe fermentations
Acetone-butanol-ethanol fermentation
Clostridium beijerinckii
Immobilization carriers
Napier grass
Pre-Treatment
Surface area measurement
Cell immobilization
Acetone
Butenes
Cells
Fermentation
Porous Materials