Publication:
Characterization of thermophilic halotolerant Aeribacillus pallidus TD1 from tao dam hot spring, Thailand

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Issued Date

2011

Resource Type

Article

File Type

application/pdf

ISSN

14220067

DOI

10.3390/ijms12085294

Other identifier(s)

2-s2.0-80052185258

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

International Journal of Molecular Sciences. Vol 12, No.8 (2011), p.5294-5303

Suggested Citation

Yasawong M., Areekit S., Pakpitchareon A., Santiwatanakul S., Chansiri K. Characterization of thermophilic halotolerant Aeribacillus pallidus TD1 from tao dam hot spring, Thailand. International Journal of Molecular Sciences. Vol 12, No.8 (2011), p.5294-5303. doi:10.3390/ijms12085294 Retrieved from: https://hdl.handle.net/20.500.14740/7272

Title

Characterization of thermophilic halotolerant Aeribacillus pallidus TD1 from tao dam hot spring, Thailand

Author(s)

Yasawong M.
 Areekit S.
 Pakpitchareon A.
 Santiwatanakul S.
 Chansiri K.

Abstract

The bacterial strain TD1 was isolated from Tao Dam hot spring in Thailand. Strain TD1 was Gram positive, rod-shaped, aerobic, motile, and endospore forming. The cell was 2.0-40 μm in length and about 0.4 μm in diameter. The optimum growth occurred at 55-60 °C and at pH 7-8. Strain TD1 was able to grow on medium containing up to 10% NaCl. The DNA G+C content was 38.9 mol%. The cellular fatty acid content was mainly C16:0, which comprised 25.04% of the total amount of cellular fatty acid. 16S rDNA showed 99% identity to Aeribacillus pallidus DSM 3670 T. Bayesian tree analysis strongly supported the idea that strain TD1 is affiliated with genus Aeribacillus, as Aeribacillus pallidus strain TD1. Although the 16S rDNA of A. pallidus strain TD1 is similar to that of A. pallidus DSM 3670 T, some physiological properties and the cellular fatty acid profiles differ significantly. A. pallidus strain TD1 can produce extracellular pectate lyase, which has not been reported elsewhere for other bacterial strains in the genus Aeribacillus. A. pallidus strain TD1 may be a good candidate as a pectate lyase producer, which may have useful industrial applications. © 2011 by the authors; licensee MDPI, Basel, Switzerland.

Subject(s)

Fatty acid
 Pectate lyase
 Fatty acid
 RNA 16S
 Aeribacillus pallidus
 Article
 Bacterial cell
 Bacterial growth
 Bacterial strain
 Bacterium examination
 Bacterium identification
 Bacterium isolation
 Cell motility
 Cell structure
 Controlled study
 DNA sequence
 Enzyme activity
 Image analysis
 Nonhuman
 Nucleotide sequence
 Photometry
 Sequence analysis
 Thailand
 Thermal spring
 Thermophilic bacterium
 Bacillaceae
 Classification
 DNA base composition
 Genetics
 Isolation and purification
 Metabolism
 Microbiology
 Physiology
 Ultrastructure
 Bacteria (microorganisms)
 Posibacteria
 Bacillaceae
 Base Composition
 Fatty Acids
 Hot Springs
 RNA, Ribosomal, 16S
 Thailand

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URI

https://hdl.handle.net/20.500.14740/7272

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Scopus: Year 1983-2021

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