Publication:
Rat β3-adrenoceptor protein expression: Antibody validation and distribution in rat gastrointestinal and urogenital tissues

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Issued Date

2014

Resource Type

Article

File Type

application/pdf

ISSN

281298

DOI

10.1007/s00210-014-1039-4

Other identifier(s)

2-s2.0-84919731329

Rights Holder(s)

Scopus

Bibliographic Citation

Naunyn-Schmiedeberg's Archives of Pharmacology. Vol 387, No.11 (2014), p.1117-1127

Suggested Citation

Cernecka H., Pradidarcheep W., Lamers W.H., Schmidt M., Michel M.C. Rat β3-adrenoceptor protein expression: Antibody validation and distribution in rat gastrointestinal and urogenital tissues. Naunyn-Schmiedeberg's Archives of Pharmacology. Vol 387, No.11 (2014), p.1117-1127. doi:10.1007/s00210-014-1039-4 Retrieved from: https://hdl.handle.net/20.500.14740/6444

Title

Rat β3-adrenoceptor protein expression: Antibody validation and distribution in rat gastrointestinal and urogenital tissues

Author(s)

Cernecka H.
 Pradidarcheep W.
 Lamers W.H.
 Schmidt M.
 Michel M.C.

Abstract

β3-Adrenoceptors play important roles in the regulation of urogenital and probably gastrointestinal function. However, despite recent progress, their detection at the protein level has remained difficult due to a lack of sufficiently validated selective antibodies. Therefore, we have explored the selectivity of two antibodies for the detection of rodent β3-adrenoceptors in immunoblots and immunohistochemistry. Of two reportedly promising candidates, antibody AB15688 did not exhibit subtype selectivity in immunoblots. In contrast, the antibody Sc1473 exhibited at least some selectivity in immunoblots and more promising results in immunocytochemical and immunohistochemical stains in cells transfected with cloned β-adrenoceptor subtypes and in rat and mouse tissues. In a systematic screening of rat gastrointestinal and urogenital tissues, Sc1473 produced selective staining in the epithelial cell lining of the stomach and the urothelium of ureter and bladder. We conclude that the two tested antibodies are inappropriate or at least insufficient for immunoblotting applications, but Sc1473 appears to be useful for immunohistochemical detection of β3-adrenoceptor protein in rodent tissues. The β3-adrenoceptor protein exhibits a distinct expression pattern in the rat gastrointestinal and urogenital tract, which is at least partly in line with previously reported functional data. © 2014 Springer-Verlag Berlin Heidelberg.

Subject(s)

Ab 15688
 Antibody
 Beta 3 adrenergic receptor
 Sc 1473
 Unclassified drug
 Antibody
 Beta 3 adrenergic receptor
 Animal cell
 Animal tissue
 Article
 Bladder
 Epithelium cell
 Gastrointestinal tract
 Genotype
 Immunoblotting
 Immunocytochemistry
 Immunohistochemistry
 Mouse
 Nonhuman
 Polymerase chain reaction
 Protein expression
 Rat
 Stomach
 Urogenital system
 Urothelium
 Animal
 C57BL mouse
 CHO cell line
 Comparative study
 Cricetulus
 Female
 Gastrointestinal tract
 Genetics
 Hamster
 Immunohistochemistry
 Immunology
 Knockout mouse
 Male
 Procedures
 Urogenital system
 Wistar rat
 Animals
 Antibodies
 CHO Cells
 Cricetinae
 Cricetulus
 Female
 Gastrointestinal Tract
 Immunoblotting
 Immunohistochemistry
 Male
 Mice
 Mice, Inbred C57BL
 Mice, Knockout
 Rats
 Rats, Wistar
 Receptors, Adrenergic, beta-3
 Urogenital System

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URI

https://hdl.handle.net/20.500.14740/6444

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Scopus: Year 1983-2021

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