Publication: Utilization of oil palm decanter cake for valuable laccase and manganese peroxidase enzyme production from a novel white-rot fungus, Pseudolagarobasidium sp. PP17-33
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Issued Date
2019
Resource Type
File Type
application/pdf
ISSN
2190572X
Other identifier(s)
2-s2.0-85074158727
Rights Holder(s)
Scopus
Bibliographic Citation
3 Biotech. Vol 9, No.11 (2019)
Suggested Citation
Thamvithayakorn P., Phosri C., Pisutpaisal N., Krajangsang S., Whalley A.J.S., Suwannasai N. Utilization of oil palm decanter cake for valuable laccase and manganese peroxidase enzyme production from a novel white-rot fungus, Pseudolagarobasidium sp. PP17-33. 3 Biotech. Vol 9, No.11 (2019). doi:10.1007/s13205-019-1945-8 Retrieved from: https://hdl.handle.net/20.500.14740/5091
Abstract
Oil palm decanter cake (OPDC) in the current study was converted to valuable products as laccase and manganese peroxidase (MnP) by an undescribed strain of the white-rot fungus, Pseudolagarobasidium sp. PP17-33. The optimization to enhance the production of enzymes through solid-state fermentation was performed using Plackett–Burman design and response surface methodology. The highest observed laccase was 5.841 U/gds and observed MnP was 5.156 U/gds, which enhanced yield by 2.59-fold and 1.94-fold from the non-optimization. The optimized medium (mg/g of OPDC) consisted of 0.852 mg CuSO4·5H2O, 13.512 mg glucose, 2 mg yeast extract, 0.2 mg KH2PO4, 1.5 mg MgSO4·7H2O, 0.01 mg FeSO4·7H2O, 0.15 mg MnSO4·H2O, 0.01 mg ZnSO4·7H2O and 0.3 mg Tween 80 (pH 5.0) when incubated at 30 °C for 7 days. The most significant variables of laccase and MnP productions were CuSO4·5H2O and glucose concentrations. This study is the first to report on the production of ligninolytic enzymes from OPDC waste using white-rot fungi. In addition, five different white-rot fungi, Coriolopsis aspera, C. retropicta, Dentipellis parmastoi, Nigroporus vinosus and Tyromyces xuchilensis, are newly observed producers of ligninolytic enzymes in Thailand. The results obtained from this study are significant not only for agro-industrial waste management but also for value-added enzyme production. © 2019, King Abdulaziz City for Science and Technology.
Subject(s)
Laccase
Manganese peroxidase
Article
Biodegradation
Coriolopsis aspera
Coriolopsis retropicta
Decolorization
Dentipellis parmastoi
Elaeis
Enzyme specificity
Enzyme synthesis
Fungus identification
Fungus isolation
Gene expression
Mycelial growth
Nigroporus vinosus
Nonhuman
Nucleotide sequence
Polymerase chain reaction
Polyporales
Priority journal
Process optimization
Pseudolagarobasidium
Screening
Sequence analysis
Solid state fermentation
Tyromyces xuchilensis
Validation process
White rot fungus
Manganese peroxidase
Article
Biodegradation
Coriolopsis aspera
Coriolopsis retropicta
Decolorization
Dentipellis parmastoi
Elaeis
Enzyme specificity
Enzyme synthesis
Fungus identification
Fungus isolation
Gene expression
Mycelial growth
Nigroporus vinosus
Nonhuman
Nucleotide sequence
Polymerase chain reaction
Polyporales
Priority journal
Process optimization
Pseudolagarobasidium
Screening
Sequence analysis
Solid state fermentation
Tyromyces xuchilensis
Validation process
White rot fungus