Publication:
Screening of potential tropical fruits in protecting endothelial dysfunction in vitro

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

16546628

DOI

10.29219/fnr.v65.7807

Other identifier(s)

2-s2.0-85114649146

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Food and Nutrition Research. Vol 65, No. (2021)

Suggested Citation

Wattanapitayakul S.K., Kunchana K., Jarisarapurin W., Chularojmontri L. Screening of potential tropical fruits in protecting endothelial dysfunction in vitro. Food and Nutrition Research. Vol 65, No. (2021). doi:10.29219/fnr.v65.7807 Retrieved from: https://hdl.handle.net/20.500.14740/4470

Title

Screening of potential tropical fruits in protecting endothelial dysfunction in vitro

Author(s)

Wattanapitayakul S.K.
 Kunchana K.
 Jarisarapurin W.
 Chularojmontri L.

Abstract

Background: High consumption of antioxidant-rich fruits and vegetables reduces the endothelial damage in-volved in cardiovascular disease pathogenesis. Objective: To evaluate the phytochemical content, antioxidant and scavenging activities (FRAP, ORAC, OH•, HOCl, H2 O2, and O2− ), endothelial H2 O2-cytoprotective effect, nitric oxide (NO) release activation potential, and endothelial wound healing properties of 10 tropical fruits, comprising pineapple, sugar apple, papaya fruit, longan, mangosteen, lychee, langsat, mango, rambutan, and guava. Design: Experimental study. The experiments were conducted in vitro using endothelial cell line EA.hy926. Results: The high performance liquid chromatography (HPLC) phytochemical analysis indicated the pres-ence of gallic acid and quercetin in all fruits, along with the overall absence of ellagic acid. Chlorogenic acid was only detected in three fruits, that is, pineapple, ripe papaya, and guava. The antioxidant and scavenging activities of all fruits were concentration-dependent. Only the H2 O2 scavenging activity exhibited broad pos-itive associations with other ROS-scavenging activities. Sugar apple and unripe papaya induced a significant reduction in H2 O2-induced cell death in endothelial cells while pineapple, sugar apple, longan, and langsat activated NO release. Discussion: All the studied tropical fruits contained bioactive phytoantioxidants with wide ranges of antiox-idant capacity and scavenging activities. The endothelial functional tests were relevant to the screening for fruits that may benefit cardiovascular health. Among the four fruits that promoted endothelial wound closure, only sugar apple and unripe papaya induced cell migration and vascular capillary-like tube formation. Conclusion: Sugar apple and unripe papaya are potential functional fruits that can protect against oxidative cell death and enhance endothelial wound healing. © 2021 Wattanapitayakul et al.T.

Subject(s)

Ananas comosus extract
 Annona squamosa extract
 Antioxidant
 Ascorbic acid
 Carica papaya extract
 Chlorogenic acid
 Dimocarpus longan extract
 Gallic acid
 Garcinia mangostanaextract
 Guava extract
 Lansium domesticum extract
 Litchi chinensis extract
 Mangifera indica extract
 Nephelium lappaceum extract
 Nitric oxide
 Nutraceutical
 Plant extract
 Quercetin
 Trolox C
 Unclassified drug
 Angiogenesis
 Annona squamosa
 Antioxidant activity
 Article
 Cell migration
 Chemical composition
 Concentration response
 Controlled study
 Dimocarpus longan
 Drug screening
 EA.hy 926 cell line
 Endothelial dysfunction
 Ferric reducing antioxidant power assay
 Fruit
 Functional food
 Garcinia mangostana
 High performance liquid chromatography
 Human
 Human cell
 Hydrogen peroxide scavenging assay
 Hydroxyl radical scavenging assay
 IC50
 In vitro study
 Lansium domesticum
 Lychee
 Nephelium lappaceum
 Oxidative stress
 Oxygen radical absorbance capacity
 Pineapple
 Transwell assay
 Wound healing assay

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URI

https://hdl.handle.net/20.500.14740/4470

Collections

Scopus: Year 1983-2021

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