Publication: Anti-metastatic effect of rhodomyrtone from Rhodomyrtus tomentosa on human skin cancer cells
1
0
Issued Date
2017
Resource Type
File Type
application/pdf
ISSN
10196439
Other identifier(s)
2-s2.0-85013067844
Rights Holder(s)
Scopus
Bibliographic Citation
International Journal of Oncology. Vol 50, No.3 (2017), p.1035-1043
Suggested Citation
Tayeh M., Nilwarangoon S., Mahabusarakum W., Watanapokasin R. Anti-metastatic effect of rhodomyrtone from Rhodomyrtus tomentosa on human skin cancer cells. International Journal of Oncology. Vol 50, No.3 (2017), p.1035-1043. doi:10.3892/ijo.2017.3845 Retrieved from: https://hdl.handle.net/20.500.14740/4238
Abstract
This study focused on the inhibitory effect of rhodomyrtone, a bioactive compound isolated from the leaves of Rhodomyrtus tomentosa (Aiton) Hassk., on cancer metastasis in epidermoid carcinoma A431 cells and on the verification of the underlying related molecular mechanisms of this event. We demonstrated that rhodomyrtone at the subcytotoxic concentration (0.5 and 1.5 μg/ml) exhibited pronounced inhibition of cancer metastasis by reducing cell migration, cell adhesive ability and cell invasion of A431 cells in a dose-dependent manner. Data demonstrated that rhodomyrtone could inhibit the focal adhesion kinase (FAK) and phosphorylation of protein kinase B (AKT), c-Raf, extracellular signal-regulated kinase 1/2 (ERK1/2) and p38 MAPK involved in the downregulation the enzyme activities and protein expression of matrix metalloproteinase-2 (MMP-2) and MMP-9. Moreover, we found that rhodomyrtone increased the expression of TIMP-1 and TIMP-2, which are inhibitors of MMP-9 and MMP-2, respectively. Rhodomyrtone also inhibited the expression of NF-κB and phosphorylation of NF-κB in a dosedependent manner. These results suggested that rhodomyrtone inhibited A431 cell metastasis by reducing MMP-2/9 activities and expression through inhibiting ERK1/2, p38 and FAK/ Akt signaling pathways via NF-κB activities. This finding suggested that rhodomyrtone may be a novel antimetastasis agent for treatment of skin cancer cells.
Subject(s)
Antimetastatic agent
C Raf protein
Focal adhesion kinase
Gelatinase A
Gelatinase B
Immunoglobulin enhancer binding protein
Mitogen activated protein kinase 1
Mitogen activated protein kinase 3
Mitogen activated protein kinase p38
Protein kinase B
Raf protein
Rhodomyrtone
Tissue inhibitor of metalloproteinase 1
Tissue inhibitor of metalloproteinase 2
Transcription factor AP 1
Unclassified drug
Antineoplastic agent
Focal adhesion kinase
Gelatinase A
Gelatinase B
Immunoglobulin enhancer binding protein
Mitogen activated protein kinase
Mitogen activated protein kinase p38
MMP2 protein, human
MMP9 protein, human
Plant medicinal product
Protein kinase B
Raf protein
Rhodomyrtone
TIMP1 protein, human
TIMP2 protein, human
Tissue inhibitor of metalloproteinase 1
Tissue inhibitor of metalloproteinase 2
Xanthone derivative
A431 cell line
Article
Cancer inhibition
Cell adhesion
Cell invasion
Cell migration
Controlled study
Down regulation
Enzyme activity
Enzyme phosphorylation
Human
Human cell
Metastasis inhibition
Myrtaceae
Plant leaf
Protein expression
Rhodomyrtus tomentosa
Signal transduction
Skin cancer cell line
Upregulation
Antagonists and inhibitors
Biosynthesis
Cell motion
Chemistry
Drug effects
Metabolism
Neoplasm Metastasis
Pathology
Phosphorylation
Skin Neoplasms
Tumor cell line
Tumor invasion
Antineoplastic Agents
Cell Adhesion
Cell Line, Tumor
Cell Movement
Extracellular Signal-Regulated MAP Kinases
Focal Adhesion Protein-Tyrosine Kinases
Humans
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Myrtaceae
Neoplasm Invasiveness
Neoplasm Metastasis
NF-kappa B
P38 Mitogen-Activated Protein Kinases
Phosphorylation
Plant Preparations
Proto-Oncogene Proteins c-akt
Proto-Oncogene Proteins c-raf
Skin Neoplasms
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2
Xanthones
C Raf protein
Focal adhesion kinase
Gelatinase A
Gelatinase B
Immunoglobulin enhancer binding protein
Mitogen activated protein kinase 1
Mitogen activated protein kinase 3
Mitogen activated protein kinase p38
Protein kinase B
Raf protein
Rhodomyrtone
Tissue inhibitor of metalloproteinase 1
Tissue inhibitor of metalloproteinase 2
Transcription factor AP 1
Unclassified drug
Antineoplastic agent
Focal adhesion kinase
Gelatinase A
Gelatinase B
Immunoglobulin enhancer binding protein
Mitogen activated protein kinase
Mitogen activated protein kinase p38
MMP2 protein, human
MMP9 protein, human
Plant medicinal product
Protein kinase B
Raf protein
Rhodomyrtone
TIMP1 protein, human
TIMP2 protein, human
Tissue inhibitor of metalloproteinase 1
Tissue inhibitor of metalloproteinase 2
Xanthone derivative
A431 cell line
Article
Cancer inhibition
Cell adhesion
Cell invasion
Cell migration
Controlled study
Down regulation
Enzyme activity
Enzyme phosphorylation
Human
Human cell
Metastasis inhibition
Myrtaceae
Plant leaf
Protein expression
Rhodomyrtus tomentosa
Signal transduction
Skin cancer cell line
Upregulation
Antagonists and inhibitors
Biosynthesis
Cell motion
Chemistry
Drug effects
Metabolism
Neoplasm Metastasis
Pathology
Phosphorylation
Skin Neoplasms
Tumor cell line
Tumor invasion
Antineoplastic Agents
Cell Adhesion
Cell Line, Tumor
Cell Movement
Extracellular Signal-Regulated MAP Kinases
Focal Adhesion Protein-Tyrosine Kinases
Humans
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Myrtaceae
Neoplasm Invasiveness
Neoplasm Metastasis
NF-kappa B
P38 Mitogen-Activated Protein Kinases
Phosphorylation
Plant Preparations
Proto-Oncogene Proteins c-akt
Proto-Oncogene Proteins c-raf
Skin Neoplasms
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2
Xanthones