Publication:
Simultaneous and direct determination of urea and creatinine in human urine using a cost-effective flow injection system equipped with in-house contactless conductivity detector and LED colorimeter

0
0

Issued Date

2019

Resource Type

Article

File Type

application/pdf

ISSN

32670

DOI

10.1016/j.aca.2019.05.003

Other identifier(s)

2-s2.0-85065554083

Rights Holder(s)

Scopus

Bibliographic Citation

Analytica Chimica Acta. Vol 1073, (2019), p.54-61

Suggested Citation

Chaneam S., Kaewyai K., Mantim T., Chaisuksant R., Wilairat P., Nacapricha D. Simultaneous and direct determination of urea and creatinine in human urine using a cost-effective flow injection system equipped with in-house contactless conductivity detector and LED colorimeter. Analytica Chimica Acta. Vol 1073, (2019), p.54-61. doi:10.1016/j.aca.2019.05.003 Retrieved from: https://hdl.handle.net/20.500.14740/5148

Title

Simultaneous and direct determination of urea and creatinine in human urine using a cost-effective flow injection system equipped with in-house contactless conductivity detector and LED colorimeter

Author(s)

Chaneam S.
 Kaewyai K.
 Mantim T.
 Chaisuksant R.
 Wilairat P.
 Nacapricha D.

Abstract

This work presents a cost-effective and simple flow injection analysis (FIA) system for simultaneous and direct determination of urea and creatinine in human urine. The FIA system comprises two in-house detectors, a contactless conductivity detector and a light emitting diode (LED) detector. The contactless detector was built as a flow-through detection cell with axial electrodes, commonly known as capacitively coupled contactless conductivity detector (C4D) and the diode detector was fabricated based on the concept of paired emitter detector diodes (PEDD). With appropriate dilution of urine, the sample is directly injected into a stream of glycine-NaOH buffer pH 8.8 (the gas donor stream) and is carried by the carrier through a urease minicolumn for on-line enzymatic hydrolysis. The generated NH3 diffuses from the carrier stream through a porous polytetrafluoroethylene (PTFE) membrane into a stream of deionized water (the acceptor stream) leading to an increase in the signal at the C4D due to the NH3 dissolution in the water. In this system, creatinine is determined based on the Jaffé reaction by merging a stream of alkaline picrate with the gas donor stream. The change in color is detected using the PEDD equipped with two green LEDs. Under the optimum condition, the linear range of urea and creatinine were 30–240 mg L−1 and 10–500 mg L−1, with limits of detection of 9.0 mg L−1 and 0.9 mg L−1, respectively. The proposed system provides satisfactorily good precision (RSD < 3%), with sample throughput of 31 sample h−1 for the two analytes. The FIA system tolerates potential interference commonly found in human urine. The system was successfully applied and validated with selected reference methods. © 2019 Elsevier B.V.

Subject(s)

Alkalinity
 Amino acids
 Ammonia
 Body fluids
 Cost effectiveness
 Deionized water
 Diodes
 Enzymatic hydrolysis
 Light emitting diodes
 Metabolism
 Sodium hydroxide
 Urea
 Capacitively coupled contactless conductivity detector (C4D)
 Creatinine
 Flow-injection analysis
 Paired emitter detector diodes
 Urine
 Cost benefit analysis
 Ammonia
 Creatinine
 Glycine
 Politef
 Sodium hydroxide
 Urea
 Urease
 Water
 Creatinine
 Urea
 Accuracy
 Article
 Dissolution
 Electric potential
 Flow injection analysis
 Flow rate
 Gas diffusion
 Human
 Hydrolysis
 In house contactless conductivity detector
 Light emitting diode colorimeter
 PH
 Priority journal
 Urinalysis
 Colorimetry
 Economics
 Electric conductivity
 Flow injection analysis
 Urine
 Colorimetry
 Creatinine
 Electric Conductivity
 Flow Injection Analysis
 Humans
 Urea

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/5148

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By