Publication:
Epidemiological, clinical and virological characteristics of influenza B virus from patients at the hospital tertiary care units in Bangkok during 2011-2014

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Issued Date

2016

Resource Type

Article

File Type

application/pdf

ISSN

19326203

DOI

10.1371/journal.pone.0158244

Other identifier(s)

2-s2.0-84978696444

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

PLoS ONE. Vol 11, No.7 (2016)

Suggested Citation

Horthongkham N., Athipanyasilp N., Pattama A., Kaewnapan B., Sornprasert S., Srisurapanont S., Kantakamalakul W., Amaranond P., Sutthent R. Epidemiological, clinical and virological characteristics of influenza B virus from patients at the hospital tertiary care units in Bangkok during 2011-2014. PLoS ONE. Vol 11, No.7 (2016). doi:10.1371/journal.pone.0158244 Retrieved from: https://hdl.handle.net/20.500.14740/5334

Title

Epidemiological, clinical and virological characteristics of influenza B virus from patients at the hospital tertiary care units in Bangkok during 2011-2014

Author(s)

Horthongkham N.
 Athipanyasilp N.
 Pattama A.
 Kaewnapan B.
 Sornprasert S.
 Srisurapanont S.
 Kantakamalakul W.
 Amaranond P.
 Sutthent R.

Abstract

Influenza B virus, which causes acute respiratory infections, has increased in prevalence in recent years. Based on the nucleotide sequence of the hemagglutinin (HA) gene, influenza B virus can be divided into two lineages, Victoria and Yamagata, that co-circulate during the influenza season. However, analysis of the potential association between the clinical and virological characteristic and the lineage of influenza B viruses isolated in Thailand was lacking. To investigate influenza B virus genetically and determine its neuraminidase (NA) inhibitor susceptibility phenotype, a total of 6920 nasopharyngeal-wash samples were collected from patients with influenza-like illness between the years 2011 and 2014 and were screened for influenza B virus by real-time PCR. Of these samples, 3.1% (216/6920) were confirmed to contain influenza B viruses, and 110 of these influenza viruses were randomly selected for nucleotide sequence analysis of the HA and NA genes. Phylogenetic analysis of the HA sequences showed clustering into various clades: Yamagata clade 3 (11/110, 10%), Yamagata clade 2 (71/110, 64.5%), and Victoria clade 1 (28/110, 25.5%). The analysis of clinical characteristic demonstrated that the Victoria lineage was significantly associated with the duration of hospitalization, number of deceased cases, pneumonia, secondary bacterial infection and underlying disease. When combined with phylogenetic analysis of the NA sequences, four samples showed viruses with reassortant sequences between the Victoria and Yamagata lineages. Statistical analysis of the clinical outcomes and demographic data for the reassortant strains did not differ from those of the other strains in circulation. Oseltamivir-resistant influenza B viruses were not detected. Our findings indicated the co-circulation of the Victoria and Yamagata lineages over the past four cold seasons in Bangkok. We also demonstrated differences in the clinical symptoms between these lineages. © 2016 Horthongkham et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Subject(s)

Oseltamivir
 Virus hemagglutinin
 Virus sialidase
 Influenza virus hemagglutinin
 Oseltamivir
 Sialidase
 Adolescent
 Aged
 Amino acid substitution
 Animal cell
 Antiviral resistance
 Article
 Child
 Controlled study
 Female
 Gene amplification
 Gene mutation
 Gene sequence
 Genetic variability
 HA gene
 Human
 Influenza B virus
 Major clinical study
 Male
 Mutational analysis
 NA gene
 Newborn
 Nonhuman
 Nucleotide sequence
 Phylogenetic tree
 Phylogeny
 Prevalence
 Real time polymerase chain reaction
 Sequence analysis
 Tertiary care center
 Thailand
 Virus detection
 Adult
 Antagonists and inhibitors
 Cluster analysis
 Dna mutational analysis
 Genetics
 Infant
 Influenza, Human
 Middle aged
 Phenotype
 Preschool child
 Tertiary care center
 Virology
 Young adult
 Adolescent
 Adult
 Aged
 Child
 Child, Preschool
 Cluster Analysis
 DNA Mutational Analysis
 Female
 Hemagglutinin Glycoproteins, Influenza Virus
 Humans
 Infant
 Infant, Newborn
 Influenza B virus
 Influenza, Human
 Male
 Middle Aged
 Neuraminidase
 Oseltamivir
 Phenotype
 Phylogeny
 Real-Time Polymerase Chain Reaction
 Tertiary Care Centers
 Thailand
 Young Adult

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URI

https://hdl.handle.net/20.500.14740/5334

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Scopus: Year 1983-2021

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