Publication: Heterologous expression and antimicrobial activity of OsGASR3 from rice (Oryza sativa L.)
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Issued Date
2018
Resource Type
File Type
application/pdf
ISSN
1761617
Other identifier(s)
2-s2.0-85044599217
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of Plant Physiology. Vol 224-225, (2018), p.95-102
Suggested Citation
Boonpa K., Tantong S., Weerawanich K., Panpetch P., Pringsulaka O., Yingchutrakul Y., Roytrakul S., Sirikantaramas S. Heterologous expression and antimicrobial activity of OsGASR3 from rice (Oryza sativa L.). Journal of Plant Physiology. Vol 224-225, (2018), p.95-102. doi:10.1016/j.jplph.2018.03.013 Retrieved from: https://hdl.handle.net/20.500.14740/5650
Abstract
According to an in silico analysis, OsGASR3 (LOC_Os03g55290) from rice (Oryza sativa L.) was predicted to be involved in plant defense mechanisms. A semi-quantitative reverse transcription polymerase chain reaction assay revealed that OsGASR3 is highly expressed in the inflorescences of Thai jasmine rice (O. sativa L. subsp. indica ‘KDML 105′). To characterize the biological activity of OsGASR3, we produced an OsGASR3–glutathione S-transferase fusion protein in Escherichia coli Rosetta-gami (DE3) cells for a final purified recombinant OsGASR3 yield of 0.65 mg/L. The purified OsGASR3 inhibited the hyphal growth of Fusarium oxysporum f.sp. cubense and Helminthosporium oryzae at a relatively low concentration (7.5 μg/mL). Furthermore, OsGASR3 exhibited in planta inhibitory activity against Xanthomonas campestris, suggesting its involvement in defense mechanisms, in addition to its previously reported functions affecting growth and development. These observations indicate that recombinant OsGASR3 may be useful for protecting agriculturally important crops against pathogenic microbes. © 2018 Elsevier GmbH