Publication:
Polyclonal antibodies specific for VP1 and VP3 capsid proteins of Taura syndrome virus (TSV) produced via gene cloning and expression

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Issued Date

2006

Resource Type

Article

File Type

application/pdf

ISSN

1775103

DOI

10.3354/dao069249

Other identifier(s)

2-s2.0-33646505325

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Diseases of Aquatic Organisms. Vol 69, (2006), p.249-253

Suggested Citation

Chaivisuthangkura P., Tejangkura T., Rukpratanporn S., Longyant S., Sithigorngul W., Sithigorngul P. Polyclonal antibodies specific for VP1 and VP3 capsid proteins of Taura syndrome virus (TSV) produced via gene cloning and expression. Diseases of Aquatic Organisms. Vol 69, (2006), p.249-253. doi:10.3354/dao069249 Retrieved from: https://hdl.handle.net/20.500.14740/5810

Title

Polyclonal antibodies specific for VP1 and VP3 capsid proteins of Taura syndrome virus (TSV) produced via gene cloning and expression

Author(s)

Chaivisuthangkura P.
 Tejangkura T.
 Rukpratanporn S.
 Longyant S.
 Sithigorngul W.
 Sithigorngul P.

Abstract

Capsid protein genes VP1and VP3 of Taura syndrome virus (TSV) were cloned into pGEX-6P-1expression vector and transformed into Escherichia coli BL21. After induction, recombinant VP1 (rVP1) and recombinant VP3 (rVP3) were produced, purified by SDS-PAGE and used for immunization of Swiss mice for antisera production. Anti-rVP1 and anti-rVP3 antisera showed specific immunoreactivities to rVP1 and rVP3 proteins, respectively, by Western blot assay and also yielded good results for detection of TSV in various shrimp tissues by immunohistochemistry. This is the first step towards our target of preparing monoclonal antibodies specific to rVP1 and rVP3 for use in simple immuno-diagnostic test kits for TSV detection and identification. © Inter-Research 2006.

Subject(s)

Antibody
 Aquatic organism
 Clone
 Disease
 Gene
 Immunization
 Virus
 Decapoda (Crustacea)
 Escherichia coli
 Litopenaeus vannamei
 Miridae
 Taura syndrome virus

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URI

https://hdl.handle.net/20.500.14740/5810

Collections

Scopus: Year 1983-2021

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