Publication:
Platelet-derived growth factor-D expression in developing and mature human kidneys

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Issued Date

2002

Resource Type

Article

File Type

application/pdf

ISSN

852538

DOI

10.1046/j.1523-1755.2002.00662.x

Other identifier(s)

2-s2.0-0036434863

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Kidney International. Vol 62, No.6 (2002), p.2043-2054

Suggested Citation

Changsirikulchai S., Hudkins K.L., Goodpaster T.A., Volpone J., Topouzis S., Gilbertson D.G., Alpers C.E. Platelet-derived growth factor-D expression in developing and mature human kidneys. Kidney International. Vol 62, No.6 (2002), p.2043-2054. doi:10.1046/j.1523-1755.2002.00662.x Retrieved from: https://hdl.handle.net/20.500.14740/6829

Title

Platelet-derived growth factor-D expression in developing and mature human kidneys

Author(s)

Changsirikulchai S.
 Hudkins K.L.
 Goodpaster T.A.
 Volpone J.
 Topouzis S.
 Gilbertson D.G.
 Alpers C.E.

Abstract

Background. Platelet-derived growth factor (PDGF) is a family of growth regulatory molecules composed of sulfide-bonded dimeric structures. Two well-studied PDGF peptides (PDGF-A and PDGF-B) have been shown to mediate a wide range of biological effects. PDGF-D is a newly recognized member of the PDGF family. Initial studies of the PDGF-D gene found its expression in cells of the vascular wall, suggesting that it could participate in vascular development and pathology. However, its localization in human kidney tissues has never been studied. Methods. PDGF-D expression in fetal (N = 30) and adult (N = 25) human kidney tissues was examined by immunohistochemistry using an affinity-purified antibody raised to human PDGF-D. Antibody absorption with the immunizing peptide was employed to confirm the specificity of this antibody. PDGF-D protein and gene expression in human kidneys also were demonstrated by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR). Results. In the developing kidney, PDGF-D was first expressed by epithelial cells of comma- and S-shaped structures of the developing nephron, and most consistently in the visceral epithelial cells in the later stages of glomerular differentiation. In addition, PDGF-D could be found in mesenchymal, presumptively fibroblast cells in the interstitium of developing renal pelvis and in fetal smooth muscle cells in arterial vessels. In the adult normal kidney, PDGF-D was expressed by the visceral epithelial cells. There was persistent expression in arterial smooth muscle cells as well as in some neointimal smooth muscle cells of arteriosclerotic vessels, and expression in smooth muscle cells of vasa rectae in the medulla. PDGF-D could be identified at the basolateral membrane of some injured tubules in areas of chronic tubulointerstitial injury routinely encountered in aging kidneys. Western blotting of homogenates of adult kidneys demonstrated monospecific bands at 50 kD corresponding to previously established size parameter for this protein. RT-PCR of human kidney RNA resulted in a 918 basepair band, the sequence of which corresponded to human PDGF-D (Genbank number AF336376). Conclusions. To our knowledge, these are the first studies to localize PDGF-D in human kidneys and suggest that PDGF-D may have a role in kidney development. PDGF-D was shown to bind to PDGF β receptor, which localizes to mesangial cells, parietal epithelial cells, and interstitial fibroblasts, suggesting potential paracrine interactions between those cells and the visceral epithelium.

Subject(s)

Lymphokine
 Messenger RNA
 PDGFD protein, human
 Platelet derived growth factor
 Platelet derived growth factor
 Platelet derived growth factor beta receptor
 Platelet derived growth factor D
 RNA
 Unclassified drug
 Adult
 Antibody specificity
 Article
 Chemistry
 Fetus
 Gene expression regulation
 Genetics
 Human
 Immunohistochemistry
 Immunology
 Kidney
 Molecular genetics
 Physiology
 Prenatal development
 Reverse transcription polymerase chain reaction
 Western blotting
 Basolateral membrane
 Cell differentiation
 Cell interaction
 Controlled study
 Fibroblast
 Gene expression
 Glomerulus epithelium
 Human cell
 Human tissue
 Interstitial nephritis
 Kidney development
 Kidney epithelium
 Kidney homogenate
 Kidney interstitium
 Nephron
 Nucleotide sequence
 Priority journal
 Protein expression
 Protein family
 Protein function
 Protein localization
 Smooth muscle fiber
 Adult
 Antibody Specificity
 Blotting, Western
 Fetus
 Gene Expression Regulation, Developmental
 Humans
 Immunohistochemistry
 Kidney
 Lymphokines
 Molecular Sequence Data
 Platelet-Derived Growth Factor
 Reverse Transcriptase Polymerase Chain Reaction
 RNA, Messenger

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URI

https://hdl.handle.net/20.500.14740/6829

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Scopus: Year 1983-2021

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