Publication:
Porcine reproductive and respiratory syndrome virus (PRRSV) preferentially infected the apical surface of primary endometrial cell monolayer

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Issued Date

2019

Resource Type

Article

File Type

application/pdf

ISSN

1256491

Other identifier(s)

2-s2.0-85085664117

Rights Holder(s)

Scopus

Bibliographic Citation

Thai Journal of Veterinary Medicine. Vol 49, No.4 (2019), p.401-413

Suggested Citation

Lothong M., Wattanaphansak S., Deachapunya C., Poonyachoti S. Porcine reproductive and respiratory syndrome virus (PRRSV) preferentially infected the apical surface of primary endometrial cell monolayer. Thai Journal of Veterinary Medicine. Vol 49, No.4 (2019), p.401-413. Retrieved from: https://hdl.handle.net/20.500.14740/5066

Title

Porcine reproductive and respiratory syndrome virus (PRRSV) preferentially infected the apical surface of primary endometrial cell monolayer

Author(s)

Lothong M.
 Wattanaphansak S.
 Deachapunya C.
 Poonyachoti S.

Abstract

The underlying mechanism of porcine reproductive and respiratory syndrome virus (PRRSV) causing reproductive failure and re-circulation in herds has remained unclear. Endometrial cells primarily infected with PRRSV may serve as a significant target for PRRSV eradication. Primary endometrial (PE) cells from the porcine uterus were isolated and cultivated to pursue this possibility. Immunocytochemistry analysis revealed the protein expression of classical estrogen receptors (ER-α and ER-β), but not PRRSV receptors, CD163 and sialoadhesin in PE cells. PE cells were apically/basolaterally inoculated with PRRSV type I/type II isolated from PRRSV infected lungs or mock infection. Cytopathic effects (CPE) and PRRSV-GP5 positive cells were detected in PE cells incubated with PRRSV inoculum (107 TCID50/ml) beginning at 4 days post inoculation (dpi). Only apical inoculation produced effects, suggesting route dependence of PRRSV infectivity in PE cells (p<0.05). PRRSV type II produced overall effects i.e., CPE, PRRSV-GP5 positive cells and a viral load higher than type I (p<0.05) during 2-6 dpi. In accordance with these effects, the tissue epithelial resistance (TER) of type II inoculated PE cells was lower than that of mock or type I inoculated cells (p<0.05). In addition, all the PE cells and media samples collected from PRRSV-inoculated PE cells persistently revealed PRRSVGP5 protein and viral copies (102-108 TCID50/ml) accessed by infecting MARC-145 cells. These findings provided the first evidence that PE cells can be directly infected with PRRSV, favorably by type II at the apical side. However, all PRRSV contaminated PE cells persistently carry the progeny virus. © 2019 Chulalongkorn University Printing House. All rights reserved.

Subject(s)

CD163 antigen
 Estrogen receptor alpha
 Estrogen receptor beta
 Sialoadhesin
 Animal cell
 Apical surface
 Article
 Cell culture
 Cell isolation
 Cell surface
 Classical swine fever virus
 Controlled study
 Cytopathogenic effect
 Data analysis
 Endometrium cell
 Immunocytochemistry
 Immunoreactivity
 Inoculation
 MARC-145 cell line
 Nonhuman
 Pig
 Porcine reproductive and respiratory syndrome virus
 Real time reverse transcription polymerase chain reaction
 Reverse transcription polymerase chain reaction
 RNA extraction
 RNA isolation
 Transepithelial potential difference
 Transepithelial resistance
 Uterus tissue
 Virus entry
 Virus isolation
 Virus load
 Virus release
 Virus transmission

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URI

https://hdl.handle.net/20.500.14740/5066

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Scopus: Year 1983-2021

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