Publication: The Leaf Extract of Coccinia grandis (L.) Voigt Accelerated in Vitro Wound Healing by Reducing Oxidative Stress Injury
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Issued Date
2021
Resource Type
File Type
application/pdf
ISSN
19420900
Other identifier(s)
2-s2.0-85100106885
Rights
Srinakharinwirot University
Rights Holder(s)
Scopus
Bibliographic Citation
Oxidative Medicine and Cellular Longevity. Vol 2021, (2021)
Suggested Citation
Namchaiw P., Jaisin Y., Niwaspragrit C., Malaniyom K., Auvuchanon A., Ratanachamnong P. The Leaf Extract of Coccinia grandis (L.) Voigt Accelerated in Vitro Wound Healing by Reducing Oxidative Stress Injury. Oxidative Medicine and Cellular Longevity. Vol 2021, (2021). doi:10.1155/2021/3963510 Retrieved from: https://hdl.handle.net/20.500.14740/5007
Abstract
The impairment in the regulation of the physiological process in the inflammatory phase of wound healing results in oxidative stress damage, which increases the severity and extends the healing time. In this study, we aimed to evaluate the radical scavenging properties of Coccinia leaf extract and its ability to ameliorate a migration process in vitro. Coccinia is a medicinal plant that was used in ancient times for relieving insect bite itching and swelling. However, the role of Coccinia leaf extract as an antioxidant related to the process of wound healing has never been studied. In this study, we demonstrated that the leaf extract possessed antioxidant properties that acted as a proton donor to neutralize reactive oxygen species with the IC50 value of 4.85 mg/mL of the extract. It could chelate iron with the IC50 value of 21.39 mg/mL of the extract. The leaf extract protected the human fibroblasts and keratinocytes from hydrogen peroxide-induced oxidative stress by increasing cell survival rate by more than 20% in all test doses. The protective property was dose-dependently correlated with the decrease in reactive oxygen species formation. In addition, the leaf extract enhanced the cell migration rate of fibroblasts and keratinocytes up to 23% compared with vehicle control. The results suggested that Coccinia leaf extract may be a potential herb for increasing the wound healing process with its antioxidant capacity and can be used as an herbal ingredient for the utilization of skincare products. © 2021 Poommaree Namchaiw et al.
Subject(s)
Antioxidants
Cell culture
Control system synthesis
Fibroblasts
Oxidative stress
Oxygen
Reactive oxygen species
Tissue regeneration
Antioxidant capacity
Antioxidant properties
Human fibroblast
Migration process
Physiological process
Protective properties
Radical scavenging properties
Wound healing process
Plant extracts
Cell culture
Control system synthesis
Fibroblasts
Oxidative stress
Oxygen
Reactive oxygen species
Tissue regeneration
Antioxidant capacity
Antioxidant properties
Human fibroblast
Migration process
Physiological process
Protective properties
Radical scavenging properties
Wound healing process
Plant extracts