Publication:
Putative salivary protein biomarkers for the diagnosis of oral lichen planus: A case-control study

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Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

14726831

DOI

10.1186/s12903-018-0504-8

Other identifier(s)

2-s2.0-85043468446

Rights Holder(s)

Scopus

Bibliographic Citation

BMC Oral Health. Vol 18, No.1 (2018)

Suggested Citation

Talungchit S., Buajeeb W., Lerdtripop C., Surarit R., Chairatvit K., Roytrakul S., Kobayashi H., Izumi Y., Khovidhunkit S.-O.P. Putative salivary protein biomarkers for the diagnosis of oral lichen planus: A case-control study. BMC Oral Health. Vol 18, No.1 (2018). doi:10.1186/s12903-018-0504-8 Retrieved from: https://hdl.handle.net/20.500.14740/6139

Title

Putative salivary protein biomarkers for the diagnosis of oral lichen planus: A case-control study

Author(s)

Talungchit S.
 Buajeeb W.
 Lerdtripop C.
 Surarit R.
 Chairatvit K.
 Roytrakul S.
 Kobayashi H.
 Izumi Y.
 Khovidhunkit S.-O.P.

Abstract

Background: Salivary protein biomarkers for screening and diagnosis of oral lichen planus (OLP) are not well-defined. The objective of this study was to identify putative protein biomarkers for OLP using proteomic approaches. Methods: Pooled unstimulated whole saliva was collected from five OLP patients and five healthy control participants. Saliva samples were then subjected to two-dimensional gel electrophoresis, followed by mass spectrometry to identify putative protein biomarkers. Subsequently, a subset of these putative biomarkers were validated in 24 OLP patients and 24 age-matched healthy control subjects, using an enzyme-linked immunosorbent assay (ELISA). Immunoblotting analyses were then performed in 3 pairs of age- and sex-matched OLP patients and healthy controls to confirm results from the ELISA study. Results: Thirty-one protein spots were identified, corresponding to 20 unique proteins. Notably, fibrinogen fragment D and complement component C3c exhibited increased expression in OLP patients, while cystatin SA exhibited decreased expression in OLP patients, compared with healthy control subjects. ELISA analyses indicated increased expression of fibrinogen fragment D and complement component C3c, and decreased expression of cystatin SA, in the saliva of OLP patients. Statistical differences in the expression of salivary complement C3c were observed between OLP patients and healthy control subjects. Immunoblotting analyses confirmed the results of our ELISA study. Conclusion: Complement C3c, fibrinogen fragment D and cystatin SA may serve as salivary biomarkers for screening and/or diagnosis of OLP. © 2018 The Author(s).

Subject(s)

Biological marker
 Complement component C3c
 Cystatin S
 Fibrin degradation product
 Fibrinogen D fragment
 Protein
 Adult
 Aged
 Case control study
 Chemistry
 Enzyme linked immunosorbent assay
 Female
 Human
 Immunoblotting
 Lichen planus
 Male
 Mass spectrometry
 Middle aged
 Proteomics
 Saliva
 Two dimensional gel electrophoresis
 Adult
 Aged
 Biomarkers
 Case-Control Studies
 Complement C3c
 Electrophoresis, Gel, Two-Dimensional
 Enzyme-Linked Immunosorbent Assay
 Female
 Fibrin Fibrinogen Degradation Products
 Humans
 Immunoblotting
 Lichen Planus, Oral
 Male
 Mass Spectrometry
 Middle Aged
 Proteins
 Proteomics
 Saliva
 Salivary Cystatins

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URI

https://hdl.handle.net/20.500.14740/6139

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Scopus: Year 1983-2021

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