Publication:
Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80

2
0

Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

10177825

DOI

10.4014/jmb.1007.07052

Other identifier(s)

2-s2.0-78650849958

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Microbiology and Biotechnology. Vol 20, No.12 (2010), p.1664-1671

Suggested Citation

Chiewpattanakul P., Phonnok S., Durand A., Marie E., Thanomsub B.W. Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80. Journal of Microbiology and Biotechnology. Vol 20, No.12 (2010), p.1664-1671. doi:10.4014/jmb.1007.07052 Retrieved from: https://hdl.handle.net/20.500.14740/7472

Title

Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80

Author(s)

Chiewpattanakul P.
 Phonnok S.
 Durand A.
 Marie E.
 Thanomsub B.W.

Abstract

A new biosurfactant producer was isolated from palm-oilcontaminated soil and later identified through morphology and DNA sequencing as the yeast-like fungus Exophiala dermatitidis. Biosurfactant production was catalyzed by vegetable oil, supplemented with a basal medium. The culture conditions that provided the biosurfactant with the highest surface activity were found to be 5% palm oil with 0.08% NH4NO3, at a pH of 5.3, with shaking at 200 rpm, and a temperature of 30°C for a 14-day period of incubation. The biosurfactant was purified, in accordance with surfactant properties, by solvent fractionation using silica gel column chromatography. The chemical structure of the strongest surface-active compound was elucidated through the use of NMR and mass spectroscopy, and noted to be monoolein, which then went on to demonstrate antiproliferative activity against cervical cancer (HeLa) and leukemia (U937) cell lines in a dose-dependent manner. Interestingly, no cytotoxicity was observed with normal cells even when high concentrations were used. Cell and DNA morphological changes, in both cancer cell lines, were observed to be cell shrinkage, membrane blebbling, and DNA fragmentation.

Subject(s)

Ammonium nitrate
 Biosurfactant
 Cell DNA
 Glycerol oleate
 Palm oil
 Animal cell
 Antineoplastic activity
 Article
 Catalysis
 Cell membrane
 Cell strain U937
 Cell structure
 Concentration response
 Controlled study
 DNA fragmentation
 DNA structure
 Drug cytotoxicity
 Drug purification
 Drug structure
 Drug synthesis
 Exophiala
 Exophiala dermatitidis
 Fungal contamination
 Fungal morphology
 Fungal strain
 Fungus culture
 Fungus identification
 HeLa cell
 Human
 Human cell
 Incubation time
 Nonhuman
 PH
 Antibiotics, Antineoplastic
 Antineoplastic Agents
 Cell Line, Tumor
 Chromatography, Liquid
 Culture Media
 Exophiala
 Humans
 Magnetic Resonance Spectroscopy
 Molecular Structure
 Plant Oils
 Silica Gel
 Soil Microbiology
 Surface-Active Agents
 Exophiala
 Exophiala dermatitidis
 Fungi

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URI

https://hdl.handle.net/20.500.14740/7472

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Scopus: Year 1983-2021

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