Publication:
Antioxidant, aα-glucosidase inhibitory activity and sub-chronic toxicity of Derris reticulata extract: Its antidiabetic potential

2
0

Issued Date

2015

Resource Type

Article

File Type

application/pdf

ISSN

14726882

DOI

10.1186/s12906-015-0552-4

Other identifier(s)

2-s2.0-84928718716

Rights Holder(s)

Scopus

Bibliographic Citation

BMC Complementary and Alternative Medicine. Vol 15, No.1 (2015)

Suggested Citation

Kumkrai P., Weeranantanapan O., Chudapongse N. Antioxidant, aα-glucosidase inhibitory activity and sub-chronic toxicity of Derris reticulata extract: Its antidiabetic potential. BMC Complementary and Alternative Medicine. Vol 15, No.1 (2015). doi:10.1186/s12906-015-0552-4 Retrieved from: https://hdl.handle.net/20.500.14740/5991

Title

Antioxidant, aα-glucosidase inhibitory activity and sub-chronic toxicity of Derris reticulata extract: Its antidiabetic potential

Author(s)

Kumkrai P.
 Weeranantanapan O.
 Chudapongse N.

Abstract

Background: Antidiabetic activity of Derris reticulata extract on alloxan-induced diabetic rats has been reported. The extract was found to lower blood glucose and inhibit intestinal glucose absorption. The aim of this study was to further investigate mechanisms underlying the antihyperglycemic activity of D. reticulata extract in vitro. Methods: The aqueous extract was obtained from D. reticulata stem. Phytochemical screening, total phenolic, and flavanoid contents were examined. ABTS and DPPH scavenging assays, and FRAP method were used to determine in vitro antioxidant activities. Measurement of cell viability on alloxan-induced cellular damage was performed in the insulin-secreting RINm5F cells by MTT assay. The effects of the extract on aα-glucosidase activity and insulin release were studied. In addition, sub-chronic toxicity test in rats was also conducted. Results: The results revealed that the extract, which consisted of terpenoids, saponins, tannins and flavonoids, possessed moderate radical scavenging activities. Pre-treatment of RINm5F cells with the extract was also found to exert moderate, but significant, in vitro protection against alloxan, an oxidative stress producing agent. Unlike glibenclamide, the extract did not stimulate insulin secretion. However, the extract was found to inhibit aα-glucosidase activity similar to acarbose. It was found that in sub-chronic toxicity studies D. reticulata extract did not cause mortality or produce any remarkable haematological, biochemical and histopathological adverse effects in rats. Conclusions: The data suggest that the possible mechanisms underlying antihyperglycemic activity of D. reticulata extract are cytoprotective effect on pancreatic cells, presumably by its antioxidant activity, and inhibition of aα-glucosidase. Sub-chronic toxicity study also provides scientific evidence to corroborate the safety of this plant as an alternative antidiabetic agent. © 2015 Kumkrai et al.; licensee BioMed Central.

Subject(s)

1,1 diphenyl 2 picrylhydrazyl
 2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid)
 Acarbose
 Alloxan
 Alpha glucosidase
 Anthraquinone derivative
 Antidiabetic agent
 Antioxidant
 Ascorbic acid
 Cardiac glycoside
 Derris reticulata extract
 Flavanoid
 Flavonoid
 Glibenclamide
 Insulin
 Phenol derivative
 Plant extract
 Saponin derivative
 Tannin derivative
 Terpenoid derivative
 Unclassified drug
 Water
 Acarbose
 Alpha glucosidase
 Antidiabetic agent
 Antineoplastic agent
 Antioxidant
 Flavonoid
 Glibenclamide
 Glucose blood level
 Glycosidase inhibitor
 Insulin
 Phenol derivative
 Plant extract
 Tannin derivative
 Alloxan-induced diabetes mellitus
 Animal cell
 Animal experiment
 Animal model
 Animal tissue
 Antidiabetic activity
 Antioxidant activity
 Article
 Biochemistry
 Blood toxicity
 Cell damage
 Cell viability
 Chemical composition
 Chronic toxicity
 Controlled study
 Derris
 Derris reticulata
 Drug fatality
 Drug isolation
 Drug screening
 Enzyme activity
 Enzyme inhibition
 Female
 Fluorescence recovery after photobleaching
 Histopathology
 In vitro study
 Insulin release
 Male
 Nonhuman
 Oxidative stress
 Phytochemistry
 Rat
 RINm5f cell line
 Adverse effects
 Animal
 Blood
 Chemistry
 Diabetes Mellitus, Experimental
 Drug effects
 Glucose blood level
 Metabolism
 Pancreas islet beta cell
 Phytotherapy
 Wistar rat
 Acarbose
 Alloxan
 Alpha-Glucosidases
 Animals
 Antineoplastic Agents, Phytogenic
 Antioxidants
 Blood Glucose
 Derris
 Diabetes Mellitus, Experimental
 Female
 Flavonoids
 Glyburide
 Glycoside Hydrolase Inhibitors
 Hypoglycemic Agents
 Insulin
 Insulin-Secreting Cells
 Male
 Phenols
 Phytotherapy
 Plant Extracts
 Rats, Wistar
 Tannins

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https://hdl.handle.net/20.500.14740/5991

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