Publication:
Development of gold nanoparticles modified screen-printed carbon electrode for the analysis of thiram, disulfiram and their derivative in food using ultra-high performance liquid chromatography

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Issued Date

2015

Resource Type

Article

File Type

application/pdf

ISSN

399140

DOI

10.1016/j.talanta.2014.09.020

Other identifier(s)

2-s2.0-84908180813

Rights Holder(s)

Scopus

Bibliographic Citation

Talanta. Vol 132, (2015), p.416-423

Suggested Citation

Charoenkitamorn K., Chailapakul O., Siangproh W. Development of gold nanoparticles modified screen-printed carbon electrode for the analysis of thiram, disulfiram and their derivative in food using ultra-high performance liquid chromatography. Talanta. Vol 132, (2015), p.416-423. doi:10.1016/j.talanta.2014.09.020 Retrieved from: https://hdl.handle.net/20.500.14740/6185

Title

Development of gold nanoparticles modified screen-printed carbon electrode for the analysis of thiram, disulfiram and their derivative in food using ultra-high performance liquid chromatography

Author(s)

Charoenkitamorn K.
 Chailapakul O.
 Siangproh W.

Abstract

For the first time, gold nanoparticles (AuNPs) modified screen-printed carbon electrode (SPCE) was developed as working electrode in ultra-high performance liquid chromatography (UHPLC) coupled with electrochemical detection (UHPLC-ED) for simultaneous determination of thiram, disulfiram, and N,N-diethyl-N',N'-dimethylthiuram disulfide, their derivative compound. The separation was performed in reversed-phase mode using C18 column, mobile phase consisting of 55:45 (v/v) ratio of 0.05 M phosphate buffer solution (pH 5) and acetonitrile at a flow rate of 1.5 mL min-1. For the detection part, the amperometric detection was chosen with a detection potential of 1.2 V vs. Ag/AgCl. Under the optimal conditions, the good linear relationship was obtained in the range of 0.07-15, 0.07-12, and 0.5-15 μg mL-1 (correlation coefficient more than 0.9900) for thiram, N,N-diethyl-N',N'-dimethylthiuram disulfide, and disulfiram, respectively. The limits of detection (LODs) of thiram, N,N-diethyl-N',N'-dimethylthiuram disulfide, and disulfiram were 0.022, 0.023, and 0.165 μg mL-1, respectively. Moreover, this method was successfully applied for the detection of these compounds in real samples (apple, grape and lettuce) with the recoveries ranging from 94.3% to 108.8%. To validate this developed method, a highly quantitative agreement was clearly observed compared to standard UHPLC-UV system. Therefore, the proposed electrode can be effectively used as an alternative electrode in UHPLC-ED for rapid, selective, highly sensitive, and simultaneous determination of thiram, disulfiram, and N,N-diethyl-N',N'-dimethylthiuram disulfide. © 2014 Elsevier B.V.

Subject(s)

Carbon
 Carbon disulfide
 Chemical detection
 Fiber optic sensors
 Fungicides
 Gold nanoparticles
 High performance liquid chromatography
 Liquids
 Metal nanoparticles
 Correlation coefficient
 Dithiocarbamates
 ELectrochemical detection
 Phosphate buffer solutions
 Screen-printed carbon electrodes
 Simultaneous determinations
 Ultra high performance liquid chromatography (UHPLC)
 Ultra-high performance liquid chromatographies
 Electrochemical electrodes
 Carbon
 Dimethylthiuram disulfide
 Disulfiram
 Fungicide
 Gold
 Metal nanoparticle
 Thiocarbamic acid derivative
 Thiram
 Analysis
 Chemistry
 Electrochemical analysis
 Electrode
 Food contamination
 High performance liquid chromatography
 Human
 Lettuce
 Limit of detection
 Malus
 Procedures
 Vitis
 Carbon
 Chromatography, High Pressure Liquid
 Disulfiram
 Electrochemical Techniques
 Electrodes
 Food Contamination
 Fungicides, Industrial
 Gold
 Humans
 Lettuce
 Limit of Detection
 Malus
 Metal Nanoparticles
 Thiocarbamates
 Thiram
 Vitis

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URI

https://hdl.handle.net/20.500.14740/6185

Collections

Scopus: Year 1983-2021

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