Publication:
Evaluation of an ethnopharmacologically selected Bhutanese medicinal plants for their major classes of Phytochemicals and biological activities

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Issued Date

2011

Resource Type

Article

File Type

application/pdf

ISSN

3788741

DOI

10.1016/j.jep.2011.06.032

Other identifier(s)

2-s2.0-80052032320

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Ethnopharmacology. Vol 137, No.1 (2011), p.730-742

Suggested Citation

Wangchuk P., Keller P.A., Pyne S.G., Taweechotipatr M., Tonsomboon A., Rattanajak R., Kamchonwongpaisan S. Evaluation of an ethnopharmacologically selected Bhutanese medicinal plants for their major classes of Phytochemicals and biological activities. Journal of Ethnopharmacology. Vol 137, No.1 (2011), p.730-742. doi:10.1016/j.jep.2011.06.032 Retrieved from: https://hdl.handle.net/20.500.14740/7253

Title

Evaluation of an ethnopharmacologically selected Bhutanese medicinal plants for their major classes of Phytochemicals and biological activities

Author(s)

Wangchuk P.
 Keller P.A.
 Pyne S.G.
 Taweechotipatr M.
 Tonsomboon A.
 Rattanajak R.
 Kamchonwongpaisan S.

Abstract

Ethnopharmacological relevance: As many as 229 medicinal plants have been currently used in the Bhutanese Traditional Medicine (BTM) as a chief ingredient of polyherbal formulations and these plants have been individually indicated for treating various types of infections including malaria, tumor, and microbial. We have focused our study only on seven species of these plants. Aim of the study: We aim to evaluate the antiplasmodial, antimicrobial, anti-Trypanosoma brucei rhodesiense and cytotoxicity activities of the seven medicinal plants of Bhutan selected using an ethno-directed bio-rational approach. This study creates a scientific basis for their use in the BTM and gives foundation for further phytochemical and biological evaluations which can result in the discovery of new drug lead compounds. Materials and methods: A three stage process was conducted which consisted of: (1) an assessment of a pharmacopoeia and a formulary book of the BTM for their mode of plant uses; (2) selecting 25 anti-infective medicinal plants based on the five established criteria, collecting them, and screening for their major classes of phytochemicals using appropriate test protocols; and (3) finally analyzing the crude extracts of the seven medicinal plants, using the standard test protocols, for their antiplasmodial, antimicrobial, anti-Trypanosoma brucei rhodesiense and cytotoxicity activities as directed by the ethnopharmacological uses of each plant. Results: Out of 25 medicinal plants screened for their major classes of phytochemicals, the majority contained tannins, alkaloids and flavonoids. Out of the seven plant species investigated for their biological activities, all seven of them exhibited mild antimicrobial properties, five plants gave significant in vitro antiplasmodial activities, two plants gave moderate anti-Trypanosoma brucei rhodesiense activity, and one plant showed mild cytotoxicity. Meconopsis simplicifolia showed the highest antiplasmodial activity with IC 50 values of 0.40 μg/ml against TM4/8.2 strain (a wild type chloroquine and antifolate sensitive strain) and 6.39 μg/ml against K1CB1 (multidrug resistant strain) strain. Significantly the extracts from this plant did not show any cytotoxicity. Conclusions: These findings provide the scientific basis for the use of seven medicinal plants in the BTM for the treatment of malaria, microbial infections, infectious fevers, and the Trypanosoma brucei rhodesiense infection. The results also form a good preliminary basis for the prioritization of candidate plant species for further in-depth phytochemical and pharmacological investigations toward our quest to unearth lead antiparasitic, anticancer and antimicrobial compounds. © 2011 Elsevier Ireland Ltd All rights reserved.

Subject(s)

Aconitum laciniatum extract
 Ajania nubigena extract
 Alkaloid
 Androsace strigillosa extract
 Antitrypanosomal agent
 Aster flaccidus extract
 Butea parviflora extract
 Capsella bursa pastoris extract
 Cavea tanguensis extract
 Chrysosplenium nudicaule extract
 Codonopsis bhutanica extract
 Corydalis crispa extract
 Corydalis dubia extract
 Flavonoid
 Fraxinus paxiana extract
 Gentiana urnula extract
 Knema tenuinervia extract
 Meconopsis simplicifolia extract
 Myricarea rosea extract
 Myrsine semiserrata extract
 Otochilus lancitabius extract
 Pedicularis integrifolia extract
 Pedicularis longiflora extract
 Plant extract
 Pleurospermum amabile extract
 Polygonum macrophylla extract
 Sapria himalayana extract
 Spasmolytic agent
 Unclassified drug
 Unindexed drug
 Veronica cephaloides extract
 Abscess
 Aconitum laciniatum
 African trypanosomiasis
 Ajania nubigena
 Androsace strigillosa
 Animal cell
 Anthelmintic activity
 Antibacterial activity
 Antifungal activity
 Antimicrobial activity
 Antiprotozoal activity
 Article
 Ascites
 Aster flaccidus
 Asthma
 Bark
 Benign tumor
 Bhutan
 Bile duct disease
 Blood disease
 Bloodstream infection
 Bone disease
 Bone infection
 Butea parviflora
 Capsella bursapastoris
 Cavea tanguensis
 Chemosensitivity
 Chickenpox
 Chrysosplenium nudicaule
 Codonopsis bhutanica
 Common cold
 Corydalis crispa
 Corydalis dubia
 Coughing
 Cytotoxicity test
 Dehydration
 Diarrhea
 Drug activity
 Drug screening
 Dysentery
 Dyspepsia
 Edema
 Epistaxis
 Evaluation
 Eye disease
 Fever
 Flower
 Fracture
 Fraxinus paxiana
 Gastritis
 Gentiana urnula
 Gout
 Heart disease
 Hepatitis
 Hypertension
 IC 50
 Infection
 Intoxication
 Kidney infection
 Knema tenuinervia
 Leprosy
 Limb disease
 Liver disease
 Lung infection
 Malaria
 Meconopsis simplicifolia
 Medical literature
 Medicinal plant
 Menstruation disorder
 Minimum inhibitory concentration
 Multidrug resistance
 Mumps
 Myricarea rosea
 Myrsine semiserrata
 Nephritis
 Neuralgia
 Nonhuman
 Otochilus lancitabius
 Pancreas disease
 Paresthesia
 Pedicularis integrifolia
 Pedicularis longiflora
 Phlogacanthus thyrsiformis
 Phlogacanthus thyrsiformis extract
 Plant leaf
 Pleurospermum amabile
 Polydipsia
 Polygonum macrophylla
 Rheumatic disease
 Rhizome
 Sapria himalayana
 Skin disease
 Skin tingling
 Stomach pain
 Traditional medicine
 Trypanosoma brucei
 Tuberculosis
 Ulcer
 Urinary tract obstruction
 Veronica cephaloides
 Wild type
 Wood
 Wound
 Animals
 Anti-Infective Agents
 Antimalarials
 Bhutan
 Cell Line, Tumor
 Cell Survival
 Cercopithecus aethiops
 Dose-Response Relationship, Drug
 Ethnopharmacology
 Formularies as Topic
 Humans
 Inhibitory Concentration 50
 Medicine, Traditional
 Microbial Sensitivity Tests
 Pharmacopoeias as Topic
 Plant Preparations
 Plants, Medicinal
 Trypanocidal Agents
 Trypanosoma brucei rhodesiense
 Vero Cells
 Meconopsis simplicifolia
 Trypanosoma brucei rhodesiense

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https://hdl.handle.net/20.500.14740/7253

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