Publication:
Monodon baculovirus (MBV) infects the freshwater prawn Macrobrachium rosenbergii cultivated in Thailand

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Issued Date

2010

Resource Type

Article

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application/pdf

ISSN

1681702

DOI

10.1016/j.virusres.2009.12.001

Other identifier(s)

2-s2.0-75449111107

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Virus Research. Vol 148, (2010), p.24-30

Suggested Citation

Gangnonngiw W., Laisutisan K., Sriurairatana S., Senapin S., Chuchird N., Limsuwan C., Chaivisuthangkura P., Flegel T.W. Monodon baculovirus (MBV) infects the freshwater prawn Macrobrachium rosenbergii cultivated in Thailand. Virus Research. Vol 148, (2010), p.24-30. doi:10.1016/j.virusres.2009.12.001 Retrieved from: https://hdl.handle.net/20.500.14740/7613

Title

Monodon baculovirus (MBV) infects the freshwater prawn Macrobrachium rosenbergii cultivated in Thailand

Author(s)

Gangnonngiw W.
 Laisutisan K.
 Sriurairatana S.
 Senapin S.
 Chuchird N.
 Limsuwan C.
 Chaivisuthangkura P.
 Flegel T.W.

Abstract

Field specimens of post-larvae of the giant freshwater prawn (Macrobrachium rosenbergii) from Thailand showed hepatopancreatic tubule epithelial cells that contained central, eosinophilic inclusions within enlarged nuclei and marginated chromatin. These inclusions resembled those produced by some baculoviruses prior to formation of occlusion bodies that enclose virions in a polyhedrin protein matrix. By electron microscopy, the intranuclear inclusions contained bacilliform, enveloped virions (approximately 327 ± 29 nm × 87 ± 12 nm) with evenly dense, linear nucleocapsids surrounded by trilaminar envelopes with lateral pockets containing nucleoproteinic filaments. In some cases, these were accompanied by moderately electron dense, spherical particles of approximately 20 nm diameter resembling polyhedrin subunits of occlusion bodies (OB) of a bacilliform virus of the black tiger shrimp Penaeus monodon, previously reported from Thailand and called monodon baculovirus (MBV). It is currently listed by the International Committee on Taxonomy of viruses as Penaeus monodon nucleopolyhedrovirus (PemoNPV). Two polymerase chain reaction (PCR) assays for MBV gave positive results with DNA extracts prepared from M. rosenbergii samples using the hot phenol technique. One of these assays targeted the polyhedrin gene of MBV to which the resulting amplicon showed 100% sequence identity. Presence of the Penaeus monodon virus polyhedrin gene was confirmed by in situ hybridization assays and by positive immunohistochemical reactions in one sample batch. The data revealed that MBV can be found but may rarely produce polyhedrin occlusion bodies in M. rosenbergii. © 2009 Elsevier B.V. All rights reserved.

Subject(s)

Polyhedrin
 Amplicon
 Animal tissue
 Article
 Baculovirus
 Cell migration
 Cell nucleus inclusion body
 Chromatin
 Controlled study
 Cultivated species
 Electron microscope
 Eosinophil
 Epithelium cell
 Hepatopancreas
 Histopathology
 Immunohistochemistry
 In situ hybridization
 Macrobrachium rosenbergii
 Monodon baculovirus
 Nonhuman
 Nucleotide sequence
 Particle size
 Polymerase chain reaction
 Priority journal
 Sequence analysis
 Shrimp
 Thailand
 Virion
 Virus infection
 Virus nucleocapsid
 Animals
 Baculoviridae
 Fresh Water
 Palaemonidae
 Thailand
 Viral Structural Proteins
 Macrobrachium rosenbergii
 Monodon baculovirus
 Mushroom bacilliform virus
 Nucleopolyhedrovirus
 Penaeus monodon

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URI

https://hdl.handle.net/20.500.14740/7613

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Scopus: Year 1983-2021

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