Publication: IMPROVEMENT OF HIGH AMYLOSE CONTENT IN CH1 RICE VARIETY BY MARKER ASSISTED PSEUDO-BACKCROSS BREEDING
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Issued Date
2024-01-01
Resource Type
ISSN
13011111
Scopus ID
2-s2.0-85200720033
Journal Title
Turkish Journal of Field Crops
Volume
29
Issue
1
Start Page
73
End Page
81
Rights Holder(s)
SCOPUS
Bibliographic Citation
Turkish Journal of Field Crops Vol.29 No.1 (2024) , 73-81
Suggested Citation
Sreewongchai T., Wangsawang T., Wangsawang S., Matthayatthaworn W., Kumdee O., Cho K.S. IMPROVEMENT OF HIGH AMYLOSE CONTENT IN CH1 RICE VARIETY BY MARKER ASSISTED PSEUDO-BACKCROSS BREEDING. Turkish Journal of Field Crops Vol.29 No.1 (2024) , 73-81. 81. doi:10.17557/tjfc.1431739 Retrieved from: https://hdl.handle.net/20.500.14740/20951
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Abstract
The objective of this research was to introgression of high amylose content into CH1 rice variety by using pseudo-backcrossing breeding. Crossing between CH1 and RD49 was performed to produce F1 progenies. After that, the progenies will backcross to CH1 to develop BC1F1 population. Then, the selected plants from the BC1F1 population were continuously selfed to develop BC1F2 and BC1F3 populations, respectively. For marker assisted selection, the OSR19 DNA marker that is specific to Wx gene was used for assisting the selection of plants with high amylose content in foreground selection to choose favorite genotype. Total 67 SSR markers used genetic marker assisted selection was utilized for BC1F1 and BC1F2 populations. The results showed that selection could be achieved for BC1F1 and BC1F2 plants having high amylose content and first highest ranking of genetic background similar to recurrent parent exhibited 91 and 97.8 percent, respectively. The marker assisted selection could accelerate in backcross breeding program. The BC1F3 seeds of 6 selected lines were planted in rice field for preliminary yield test. It was found that some agronomic characters (Days of 50% flowering, flag leaf length, panicle length, number of tillers per plant and harvest index) and yield of the selected lines were not statistically different from those of CH1 variety and high amylose content as donor parent. Thus, the advantage of the pseudo-backcross method is that it can save not only time and workload, but also the cost of analysis and leaping generations in the normal backcross method. Moreover, genetic similarity results in relatively similar morphological characteristics.