Publication:
The existence of opioid receptors in the cochlea of guinea pigs

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Issued Date

2006

Resource Type

Article

File Type

application/pdf

ISSN

0953816X

DOI

10.1111/j.1460-9568.2006.04810.x

Other identifier(s)

2-s2.0-33744457675

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

European Journal of Neuroscience. Vol 23, No.10 (2006), p.2701-2711

Suggested Citation

Jongkamonwiwat N., Phansuwan-Pujito P., Casalotti S.O., Forge A., Dodson H., Govitrapong P. The existence of opioid receptors in the cochlea of guinea pigs. European Journal of Neuroscience. Vol 23, No.10 (2006), p.2701-2711. doi:10.1111/j.1460-9568.2006.04810.x Retrieved from: https://hdl.handle.net/20.500.14740/5789

Title

The existence of opioid receptors in the cochlea of guinea pigs

Author(s)

Jongkamonwiwat N.
 Phansuwan-Pujito P.
 Casalotti S.O.
 Forge A.
 Dodson H.
 Govitrapong P.

Abstract

Several independent investigations have demonstrated the presence of opioid peptides in the inner ear organ of Corti and in particular in the efferent nerve fibers innervating the cochlear hair cells. However, the precise innervation pattern of opioid fibers remains to be investigated. In the present study the expression of opioid receptors and their peptides is demonstrated in young adult guinea pig cochlea. Opioid receptors are mainly expressed in hair cells of the organ of Corti and in inner and outer spiral bundles with different characteristics for each type of receptor. Co-localization studies were employed to compare the distribution of mu-, delta- and kappa-opioid receptors and their respective peptides, β-endorphin, leu-enkephalin and dynorphin. Additionally, immunostaining of synaptophysin was used in this study to identify the presynaptic site. Immunoreactivity for enkephalin and dynorphin was found in the organ of Corti. Leu-enkephalin was co-localized with synaptophysin prominently in the inner spiral bundle (ISB). Dynorphin was co-localized with synaptophysin in both inner and outer spiral bundles. Delta-opioid receptor was most prominently co-localized with its peptide in the ISB bundle. Kappa-opioid receptor was seemingly present with dynorphin in both inner and outer spiral bundles. The co-staining of both peptides and receptors with synaptophysin in the same areas suggests that some of the opioid receptors may act as auto-receptors. The results provide further evidence that opioids may function as neurotransmitters or neuromodulators in the cochlea establishing the basis for further electrophysiological and pharmacological investigations to understand better the roles of the opioid system in auditory function. © The Authors (2006).

Subject(s)

Autoreceptor
 Beta endorphin
 Delta opiate receptor
 Dynorphin
 Kappa opiate receptor
 Leucine enkephalin
 Mu opiate receptor
 Neurotransmitter
 Opiate
 Opiate receptor
 Synaptophysin
 Animal tissue
 Article
 Cochlea
 Controlled study
 Corti organ
 Electrophysiology
 Guinea pig
 Hair cell
 Hearing
 Male
 Nonhuman
 Nucleotide sequence
 Priority journal
 Protein expression
 Animals
 Cochlea
 Dynorphins
 Enkephalin, Leucine
 Guinea Pigs
 Immunohistochemistry
 Male
 Microscopy, Confocal
 Receptors, Opioid
 Receptors, Opioid, delta
 Receptors, Opioid, kappa
 Receptors, Opioid, mu
 Reverse Transcriptase Polymerase Chain Reaction
 RNA, Messenger
 Synaptophysin

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URI

https://hdl.handle.net/20.500.14740/5789

Collections

Scopus: Year 1983-2021

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